【摘要】 分别对牛冷冻胚胎的解冻液、解冻后胚胎的质量评定、解冻后的胚胎处理,及受体牛的同步发情处理方法和移植方法等方面开展研究。结果表明:(1)进口解冻液脱甘油的胚胎复苏率为76%(19/25),优于传统解冻液。(2)解冻后未作任何处理直接移植的妊娠率(68.4%),产犊率(47 37%)最高,切割未作培养的次之(妊娠率37.5%,产犊率25%,流产率33.3%),移植前切割培养的最差(妊娠率、产犊率均为9.09%);(3)受体黄体发育正常、同步发情率高达83%,妊娠率高达62 5%;(4)移植枪加套管受体妊娠率(52.9%)明显比不加套管的高。熟练的操作技术受体妊娠率较高(62.5%)。更多还原

【Abstract】 The defrost liquid of the freezing embryo, the qualification evaluation after the defrosting embryo, the disposing of the defrosting embryo, and the improvement of synchronous estrous disposing method for receptor synchro and the transplant method, etc,were studied. The results indicated that:(1)the resurrection rates of the embryo using import liquid was 76%(19/25),better than that using common defrost liquid;(2)the highest pregnancy rates and the deliverly rates of the receptor were in order of the treatment : the embryos without any handling> transplanted and cutted embryo without culturing> the cutted and cultured embryo; (3)the best synchronous pubescent rate of receptor with common progeste was 83%,the receptor pregnancy rates was 625%;(4) the pregnancy rates(52.9%) of the receptor using transplanting gun was better than that without using transplanted one, and the pregnancy of the receptor transplanted by the skilled workers was better.更多还原