【摘要】 以松墨天牛的消化生理为基础,研究了松墨天牛消化酶中纤维素酶的动力学特性;以现代酶学研究方法为主,结合生物测定技术,进一步研究了不同性质和不同浓度的酶抑制剂对松墨天牛成虫离体和活体纤维素酶酶活力的影响。结果表明,选用的酶抑制剂无论对分离提取的纤维素酶液和活体中的纤维素酶都具有显著的抑制作用。饲养试验中,经0.1% 酶抑制剂处理的饲料组5d 死亡率为75 %,ck组平均成活天数为28d。更多还原

【Abstract】 Study on dynamics characteristics of cellulase at Monochamus alternatus抯 digestive ferment demonstrated effect of cellulase inhibitor with different property and concentration on enzyme activity of isolated and vital cellulase of adult M. alternatus. The result showed that cellulase inhibitor had evident inhibition on isolated and vital cellulase. Feeding experiment showed that mortality rate of the group feed with 0.1% cellulase inhibitor topped 75% in 5 days, while the control had 28 survival days.更多还原