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铜(Ⅱ)对漆酶活性的影响
The Effect of Cu (Ⅱ)on Laccase Activity
【摘要】 以2,2-连氮-双(3-乙基苯并噻唑-6-磺酸)(ABTS)为底物,研究了Cu2+离子对漆酶活性的影响。实验结果表明,漆酶的活性受pH的影响非常大;Cu2+离子在不同的pH范围和浓度下,对漆酶的活性有不同的作用。
【Abstract】 By assaying the laccase activity wit h the substrate(ABTS)we find that the copper ion(Ⅱ)have different effect on laccase activity at different pH value and di fferent Cu 2+ concentration.When the pH value is u nder 4and the concentration is 1~10m g /L,the Cu(Ⅱ)inhabits the laccase.While the pH va lue is higher than 4.5at the concentr ation 1~10mg /L,the Cu(Ⅱ)can increase the activity of laccase.The Cu 2+ concentration 20mg /L is a turning point in all pH value.
【基金】 国家自然基金资助项目。
- 【文献出处】 纸和造纸 ,Paper and Paper Making , 编辑部邮箱 ,2003年04期
- 【分类号】Q55
- 【被引频次】33
- 【下载频次】388