【摘要】 目的　设计并合成针对人酪氨酸酶的反义寡核苷酸 ,从基因表达水平调控细胞黑素的合成 ,为色素沉着性疾病的治疗寻找新途径。方法　将培养的黑素细胞分为内皮素处理组、紫外线处理组和对照组 ,并分别加入 5′端反义核酸、3′端反义核酸、混合反义核酸或单纯脂质体 ,分别检测黑色素含量和酪氨酸酶mRNA的表达。结果　反义核酸对内皮素及紫外线照射引起的黑素细胞色素含量的增加以及TYR基因表达的增强有明显的抑制作用 ,其中 3′端反义核酸作用最强。结论　反义寡聚脱氧核苷酸对黑素细胞黑素产量和TYR的基因表达有显著的调控作用 ,3′端反义核酸的调控作用优于 5′端反义核酸。更多还原

【Abstract】 Objective Despite the causes for melanin increase, the increased gene expression of TYR is a common pathological process. Based on this viewpoint, antisense-S-Oligo of TYR was designed and synthesized to regulate synthesis of melanin in order to explore the treatment for skin pigmentation. Methods The cultured melanocytes were divided into 3 groups. The group 1 was treated with endothelin, group 2 treated with ultraviolet ray and group 3 was used as the control. In each group, the 5′ antisense-S-Oligo, the 3′ antisense-S-Oligo, the mixed antisense-S-Oligo of TYR or Dotap only was added. The melanin content and TYR gene expressions were examined. Results The 5′ antisense-S-Oligo, the 3′ antisense-S-Oligo and the mixed antisense-S-Oligo significantly inhibited the increase of melanin content and TYR gene expression, which were caused by endothelin or ultraviolet ray treatment. Of the three treatments, the 3′ antisense-S-Oligo showed the strongest effect. Conclusion Antisense-S-Oligo has significant regulating effects on TYR gene expression and melanin content. The 3′ antisense-S-Oligo is more effective than the 5′ antisense-S-Oligo.;更多还原