【摘要】 目的　探讨上皮细胞黏附分子 (EP CAM)和 β 环连蛋白 (β catenin)在子宫颈鳞癌癌变中的可能作用。方法　用免疫组化染色的方法检测 14例正常宫颈鳞状上皮、32例宫颈上皮内瘤变(CIN)、38例宫颈浸润性鳞状细胞癌标本中EP CAM、β catenin蛋白的表达情况。 结果　EP CAM在正常宫颈鳞状上皮组、CINⅠ组、CINⅡ组、CINⅢ组和宫颈鳞癌组细胞膜的过表达率分别为 0、7.1%、2 0 .0 %、6 2 .5 %和 5 5 .3% ,其中CINⅢ组和宫颈鳞癌组EP CAM的过表达率均明显高于正常组和CINⅠ组 (P <0 .0 0 1)。正常宫颈鳞状上皮未见 β catenin的异常表达 ,CINⅠ、Ⅱ、Ⅲ和鳞状细胞癌组β catenin的异常表达率分别为 2 8.6 %、4 0 .0 %、6 2 .5 %和 84 .2 % ,β catenin异常表达率随CIN分级的提高和癌变而相应增高。EP CAM的过表达与子宫颈鳞癌分化程度呈负相关 (P <0 .0 0 1)。结论　EP CAM、β catenin可能参与了子宫颈鳞状上皮的恶性转化过程 ,EP CAM的过表达、β catenin的异常表达可作为子宫颈鳞状上皮癌变的参考依据。更多还原

【Abstract】 Objective To study the expression of EP CAM, beta catenin in the carcinogenesis of squamous cell carcinoma of uterine cervix. Methods The expressions of EP CAM and beta catenin were detected with immunohistochemical stain in 14 cases of normal cervical squamous epithelium, 32 cases of cervical intraepithelial neoplasia (CIN) and 38 cases of cervical invasive squamous cell carcinoma. Results The over expression rates of EP CAM were 0, 7.1%, 20.0%, 62.5% and 55.3% for normal cervical epithelium, CINⅠ, CINⅡ, CINⅢ and carcinoma groups. The EP CAM over expression rates in CINⅢ and cervical carcinoma groups were significantly higher than those in normal epithelium and CINⅠ groups ( P <0.001). No aberrant expression of beta catenin was shown in normal cervical epithelium, while the aberrant expression rates of beta catenin in CINⅠ, CINⅡ, CINⅢ and cervical carcinoma group were 28.6%, 40.0%, 62.5% and 84.2%. The aberrant expression rate of beta catenin increased with the increase in degree of CIN and development of cervical carcinoma. The over expression rate of EP CAM was reversely related to the differentiation of cervical squamous cell carcinoma ( P <0.001).Conclusion EP CAM and beta catenin may be involved in the carcinogenesis of squamous cell carcinoma of uterine cervix. The over expression of EP CAM and aberrant expression of beta catenin may serve as markers of squamous carcinogenesis of uterine cervix. [更多还原