【摘要】 目的　探讨RASSF1基因 3种不同转录本在肺癌中的表达情况及其临床意义。方法采用RT PCR方法检测 5 1例肺癌癌组织及相应癌旁正常组织中RASSF1A、RASSF1B及RASSF1CmRNA的表达情况。结果　 (1) 3种不同转录本在癌旁正常肺组织中均表达 ;在癌组织中 ,RASSF1A及RASSF1B存在较高的表达缺失 ,其表达缺失率分别为 5 4 .9% (2 8/ 5 1)和 37.3% (19/ 5 1) ,RASSF1C则全部表达。 (2 )RASSF1A表达与肺癌淋巴结转移及病理分期有关 ,淋巴结转移者及晚期病例均存在较高的RASSF1A表达缺失 (P <0 .0 5 )。 (3)RASSF1B和RASSF1C表达与肺癌分期、组织学类型、分化程度及吸烟指数等均无显著相关性 (P >0 .0 5 )。结论　RASSF1基因 3种不同转录本在肺癌中的表达存在明显差异 ,其中转录本A与肺癌淋巴结转移及病理分期相关 ,是一新型肺癌抑癌基因。更多还原

【Abstract】 Objective To evaluate the expression of three different RASSF1 transcripts and its clinical significance in lung carcinomas. Methods The mRNA expression of RASSF1A, RASSF1B and RASSF1C was detected by RT PCR in 51 human lung cancer tissues and 51 matched normal tissues. Results 1. The mRNA expression of three RASSF1 transcripts was detectable in all non cancer tissues. However, high rate of expression loss of RASSF1A and RASSF1B existed in lung cancer tissues, which was 53.2%(2851) and 37.3%(19/51), respectively. RASSF1C was expressed in all of the tumor tissues. 2. Loss or abnormal down regulation of RASSF1A was positively related with lymph node metastasis and TNM stage ( P <0.05) and 3. RASSF1B and RASSF1C mRNA expression was not correlated with TNM stage, histological type, differentiation grade or smoking index. Conclusion There is a significant expression difference among the three RASSF1 transcripts in lung carcinoma. RASSF1A, closely associated with lymph metastasis and TNM stage of lung carcinoma, should be a new tumor suppressor gene.更多还原