【摘要】 目的　探讨硫酸基转移酶 (sulfotransferase,SULT) 1A1基因Arg2 13His多态性与中国汉族女性乳腺癌的相关性 ,及其与乳腺癌相关危险因素的相互作用。方法　采用病例 对照、PCR RFLP方法检测了 2 0 9例原发性乳腺癌患者和 4 2 6例匹配对照 ,SULT1A1基因Arg2 13His多态性分布 ,并应用多因素Logistic回归等方法分析基因型对乳腺癌的危险度OR以及与相关危险因素的交互作用系数r。结果　 (1)SULT1A1Arg/Arg、Arg/His、His/His三种基因型分布病例与对照组之间差异无显著意义 (P =0 .12 )。病例、对照His等位基因频率分别为 13.6 %和 9.5 % (P =0 .0 3) ;(2 )与Arg/Arg基因型相比 ,Arg/His、His/His危险度呈增加趋势 (P =0 .0 4 ) ,His等位基因 (Arg/His +His/His)的调整OR为 2 .6 0 (95 %CI:1.12～ 6 .0 5 ,P =0 .0 4 ) ;(3)变异等位基因His对内源性雌激素暴露效应有放大作用 (r>1) ,对某些饮食摄入致癌物质暴露效应有减弱作用 (r <1) ,两种交互作用均为相乘模型。结论　SULT1A1His等位基因与汉族女性乳腺癌的危险性相关 ,且与其他相关危险因素之间存在交互作用。更多还原

【Abstract】 Objective To assess the relationship between SULT1A1 Arg213His polymorphism and breast cancer in Hans of Chinese women, as well as the interaction of the polymorphism and related risk factor of breast cancer. Methods Distribution of Arg213His polymorphism of the SULT1A1 gene was detected by using PCR-RFLP in 209 cases of breast cancer and 426 matched healthy controls. The OR of susceptibility of SULT1A1 genotypes to breast cancer and the r value of interaction with exposure factors using multivariate logistic regression. Results ①There was no significant difference in genotype of Arg/Arg, Arg/His and His/His of SULT1A1 between case and control( P =0.12). The frequency of His allele was 13.6% and 9.5% in were determined case and control, respectively, P =0.03; ②Compared with Arg/Arg genotype, the risk of Arg/His and His/His was increased, P for trend=0.04; The OR of His allele (Arg/His+His/His) was 2.60 (95% CI : 1.12～6.05, P =0.04); ③As for the interaction between gene and related exposure factors, His allele strengthen the effect of endogenous estrogen exposure(interaction index r >1),and weaken the effect of factors related to exposure of some procarcinogen in dietary( r <1), both being multiplicative interaction model. Conclusion SULT1A1 His allele is positively associated with the risk of breast cancer, and an interaction exist between this allele and related exposure factors.更多还原