【摘要】 目的　探讨中国和澳大利亚宫颈癌患者人乳头状瘤病毒 (HPV)感染及其型别分布 ,并比较两组患者其他危险因素和临床病理特征的差异。方法　采用聚合酶链反应方法分别检测 115例中国宫颈癌患者和 79例澳大利亚宫颈癌患者癌组织中HPVDNA ,用测序法和反向核酸杂交法对HPV进行分型。并分析HPV感染与临床病理特征的关系。结果　中国组HPV阳性率为 76 .8% ,澳大利亚组为 89.9% (P =0 .0 17)。两组间HPVl6和 18型阳性率差别无显著意义 (P =0 .80 6 ) ,HPV5 8和 5 9型在中国组比澳大利亚组多见。HPV阳性率与临床分期、肿瘤组织学类型和分级 ,以及淋巴结转移等无关 ,但HPV型别与组织学类型及分级相关。澳大利亚组中晚期及非鳞癌患者较中国组多。两组患者在宫颈涂片普查次数及一些危险因素方面差异有显著意义。结论　澳大利亚组子宫颈癌患者HPV感染率比中国组明显为高 ;在中国组中HPV5 8和 5 9型是并列第 3常见的感染类型 ;HPV18和 5 9型与恶性度高的宫颈癌相关。更多还原

【Abstract】 Objective To investigate the infection rate and genotypes of HPV in cervical cancer and analyse the clinicopathological characteristics and risk factors for cervical cancer with HPV infection in Chinese and Australian patients.Methods The human papillomavirus(HPV)DNA in cervical cancer specimens from 115 Chinese women and 79 Australian women was detected by poly merase chain reaction.HPV types were determined by sequencing and reverse hybridization.The relationships between the HPV types and the clinicopathological characteristics of cervical cancer in Chinese and Australian women were analyzed.Results HPV DNA positive rate was 76.5%in the Chinese women(88/115)and 89.9%in the Australian women(71/79,P=0.017).There were no significant differences of HPV 16 and HPV 18 prevalence between the two groups(P:0.806).HPV 58 and 59 were more prevalent in Chinese women(both 6.8%)than in Australian women(1.4%and 0).In China,HPV 58 prevalence rate was significantly higher in the Hunan region than in the Guangdong region(P=0.007).The mean ages atdiagnosis was 44.24±11.31 years for Chinese and 53.08±16.54 years for Australian women (P<0.00 1).Combining the two groups,no relationship was found between HPV positivity/negativity and the FIGO stage,macroscopic features,histological type and grade of tumour,as well as pelvic lymphatic metastasis.However,HPV type was significantly correlated with the histological type(P<0.00 1)and grade of cervical tumour(P=0.028).In comparison with the Chinese group,the Australian group presented more advanced cancers,a greater proportion of endoeervical patterns,and more non-squamous cell carcinoma.There were no significant differences in grade of turnout and lymphatic node status between the two groups.Conclusion The HPV infection rate in cervical cancer patients is significantly higher among Australians than among Chinese,which may be caused by more sexual transmitted diseases and more sex partners among the former group.HPV 58 and HPV 59 tie for third common genotypes in cervical cancers in China,however,these types are uncommon in Australia.There was a significant variation in types of HPV infection among different histological type and grade of cervical tumors.Both HPV 18 and 59 appear to beassociated with the malignancy of cervical cancer.更多还原