【摘要】 目的　研究叶绿酸对反式 7,8 二羟 9,10 环氧苯并芘 (反式 BPDE)诱发的SV40 largeT抗原永生化的人支气管上皮细胞系 (16HBE)恶性转化的抑制作用。方法　在反式 BPDE诱导的细胞转化整个过程中 ,分别加入浓度为 10、5 0和 10 0 μmol/L的叶绿酸 ,应用刀豆凝集素 (ConA)凝集实验、软琼脂集落形成实验和裸鼠成瘤实验进行细胞恶性度鉴定。结果　细胞培养至 2 5代 ,发现叶绿酸和反式 BPDE共处理组细胞与反式 BPDE单独处理组细胞相比 ,前者ConA凝集时间延长 ;叶绿酸和反式 BPDE共处理组细胞在软琼脂中集落形成率分别为 7 4‰、11 4‰和 14 4‰ ,明显低于反式 BPDE单独处理组 (19 6‰ ) ,且有剂量反应关系 ;各处理组细胞均能在裸鼠体内成瘤 ,叶绿酸和BPDE共处理组在裸鼠体内生长的肿瘤体积分别为 (0 4 3± 0 13)cm3 、(0 2 2± 0 0 4 )cm3 、(0 10± 0 0 6 )cm3 ,明显小于BPDE单独处理组的 (1 71± 0 37)cm3 ,并有剂量依赖关系 ,肿瘤组织经病理学证实为低分化鳞状细胞癌。结论　体外试验显示叶绿酸具有一定的抗细胞恶变能力。更多还原

【Abstract】 Objective To study the inhibitory effect of chlorophyllin (CHL) on trans-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide (BPDE) induced malignant transformation in human bronchial epithelial cell line (16HBE). Methods 10, 50 or 100 μmol/L CHL were added into the media during the cells transformation induced by BPDE, and the malignant degree of transformed cells were identified by the ConA agglutination test and the assay for anchorage-independent growth and tumorigenicity. Results After the cells were cultured for 25 times, the time of cells agglutination in groups treated with both CHL and BPDE was increased significantly; the colony formation efficiency in soft agar in groups treated with both CHL and BPDE (7.4‰, 11.4‰ and 14.4‰) showed significant decrease (P<0.05) in dose-dependent manner, as compared with that in group treated with BPDE alone (19.6‰). Cells treated with both CHL and BPDE or BPDE alone developed tumor in nude mice, a squamous carcinoma confirmed by histopathological examination. The volume of tumor in groups treated with both CHL and BPDE (0.43±0.13) cm2, (0.22±0.04)cm2 and (0.10±0.06) cm3 was significantly smaller (P<0.05) and dose-dependent, as compared with that in the group treated with BPDE alone (1.71±0.37) cm3. Conclusion CHL showed significant antitransforming ability in human bronchial epithelial cell line induced by BPDE.更多还原