【摘要】 目的　探讨遗传标记物 c-Ha-ras基因 3′端可变串联重复序列 (variable numbers of tandemrepeats,VNTR)在妊娠滋养细胞疾病中的变化 ,以了解 DNA来源与葡萄胎恶变的关系。方法　用扩增片段长度多态性 -聚丙烯酰胺凝胶电泳方法对 c-Ha-ras基因 3′端 VNTR位点进行分析 ,将患者病变组织与其父母的 DNA扩增结果进行对照 ,以判定病变组织 DNA的来源。结果　在 15组病例中 ,仅有 2例葡萄胎组织的 DNA扩增片段全部来自父方 ;既来自父方又来自母方者有 13例 ,其中 2例来自父母双方的比例是一样的 ,11例来自父方的比例大于来自母方的比例。结论　DNA来自父母亲双方的 ,且来自父方的比例大于来自母方比例的葡萄胎组织易于发生恶变更多还原

【Abstract】 Objective To study the polymorphism of c-Ha-r as-3′-variable number of tanden repeat(VNTR) and its relationship with development of postmolar tumor. Methods The cases of hydatidiform mole(HM) were retrospectively analyzed by amplified fragm ent length polymorp hism-polyacrylamide gel electrophoresis. The DNA origin of HM was determined by comparison with the parents’ DNA amplified results. Results Amon g the samples from 15 cases, DNA from only paternal origin was found in 2 cases. DNA from both parents was in 13 cases, and of these 13 cases, 2 were found to b e had balanced DNA origin, 11 had more DNA form paternal origin than DNA from maternal origin. Conclusion The HM which has DNA from both par ents origin and predominantly from paternal origin developed to postmolar tumo r more frequently.更多还原