【摘要】 目的　解码高三尖杉酯碱 (HHT)启动白血病细胞凋亡相关蛋白。方法　在建立HHT启动HL 6 0细胞早期凋亡模型的基础上 ,分别提取未用和经HHT作用的HL 6 0细胞总蛋白 ,构建其相应的双向电泳图谱 ,应用图像扫描仪及图像分析软件获得差异蛋白斑点的信息 ,运用MALDI TOF MS分析呈显著性差异的蛋白斑点 ,将检测出的肽链质量输入蛋白质数据库获得差异蛋白质信息。结果　MHCⅠ类抗原分子、钙结合蛋白D 2 8K、氯通道蛋白 6、癌蛋白OP18、锌指蛋白HELIOS和凋亡抑制物样蛋白 2与HHT启动白血病HL 6 0细胞凋亡相关。结论　运用比较蛋白质组学技术解码HHT启动白血病细胞凋亡相关蛋白 ,将有助于揭示HHT治疗白血病的机制 ,为开发HHT相关的以诱导白血病细胞凋亡为靶位的药物先导化合物提供参考。更多还原

【Abstract】 Objective To study the related proteins of apoptosis initiation induced by homoharringtonine(HHT) in HL-60 cells. Methods After establishment of an apoptosis initiation model induced by HHT in HL-60 cells, proteins of untreated and HHT treated HL-60 cells were extracted, and the two-dimensional polyacrylamide gel electrophoresis(2-DE) maps of the extracted proteins were established by using the immobilized pH gradient(IPG)two-dimensional electrophoresis respectively. The alteration protein spots were identified with assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching. Results Proteomics analysis showed that proteins including MHC class I antigen, calbindin D-28K,chloride channel protein 6,oncoprotein 18, zinc finger protein Helios and apoptosis inhibitor like protein 2 were involved in apoptosis initiation induced by HHT. Conclusion The present study might conduce to the researches of HL-60 cells carcinogenesis and pave the way to exploit drug precursor related to HHT and initiation of apoptosis in HL-60 cells.更多还原