【摘要】 目的　研究血管紧张素Ⅱ (AngⅡ )对模拟缺血心室肌细胞内游离钙离子浓度的作用。方法　用胶原酶酶解法急性分离豚鼠心室肌细胞 ,经Fluo 3/AM负载染色后 ,用激光共聚焦扫描 ,测定荧光强度 ,反映细胞内游离钙离子浓度 ;采用低氧、无糖、高乳酸和酸中毒综合方式模拟缺血液灌流 ,造成细胞的模拟缺血 ,并在缺血的基础上观察AngⅡ对模拟缺血心室肌细胞内游离钙离子浓度的影响。结果　模拟缺血可缓慢增加心室肌细胞内游离钙浓度 ;AngⅡ能使模拟缺血细胞内游离钙浓度增高 ,并呈脉冲式变化。结论　AngⅡ能增加模拟缺血心肌细胞静息钙水平 ,导致钙超载。更多还原

【Abstract】 Objective To observe the effects of angiotensin Ⅱ(Ang Ⅱ) on intracellular calcium concentration ([Ca 2+] i) in guinea pig isolated ventricular myocytes under simulated ischemia condition.Method Using Fluo-3/AM loading and confocal microscopy, the effects of Ang Ⅱon [Ca 2+] i were observed in guinea pig isolated ventricular myocytes under simulated ischemia condition, which mimicked hypoxia,glucose deficient,high lactic acid and acidosis.Result [Ca 2+] i increased steadily during the period of simulated ischemia, while Ang II resulted in a periodic pulse-like increase in [Ca 2+] i.Conclusion Ang Ⅱ may increase [Ca 2+] i during the period of simulated ischemia which result in calcium overload.更多还原