【摘要】 目的　研究大鼠骨髓基质干细胞在体外分化为类许旺细胞的方法。　方法　采用 β 巯基乙醇、全反式视黄酸、Forskolin、碱性成纤维细胞生长因子等依次按顺序诱导。神经胶质纤维酸性蛋白、S 10 0免疫细胞化学染色鉴定、计数诱导前后不同时间的细胞阳性表达率。　结果　诱导后骨髓基质干细胞形态改变 ,胞体呈椭圆形 ,有 2～ 3个纤细的细胞突起 ,成纵形栅栏状排列 ,免疫细胞化学染色神经胶质纤维酸性蛋白表达率为 42 5 %～ 6 8 7%、S 10 0表达率为 39 6 %～ 6 0 2 %。　结论　采用 β 巯基乙醇、全反式视黄酸、Forskolin、碱性成纤维细胞生长因子、血小板源生长因子、HRG可以诱导大鼠骨髓基质干细胞在体外分化为类许旺细胞。更多还原

【Abstract】 Objective To study the protocol that rat bone marrow stromal cells (BMSCs) can be induced to differentiate into Schwann cell-like in vitro. Methods BMSCs were treated with beta-mercaptoethanol followed by retinoic acid and cultured in the presence of forskolin,basic-FGF,PDGF and heregulin. The positive percentages of GFAP,S-100 protein expression were measured by immunocytochemistry SABC staining. Results After the induction,BMSCs displayed morphologies of Schwann cell,such as ellipsoidal cell bodies and fences-like array,with two or three sligh cell processes. The positive percentages of GFAP protein expression was from 42.5% to 68.7%,S-100 was from 39.6% to 60.2%. Conclusions Rat BMSCs could be induced to Schwann cell-like by beta-mercaptoethanol,retinoic acid,forskolin,basic-FGF,PDGF and heregulin更多还原