【摘要】 目的　测定培养状态下间皮细胞的抗凝与纤溶作用的强度并与内皮细胞、成纤维细胞作比较 ,为血管假体内腔铺被细胞的选择提供依据。方法　取SD大鼠大网膜、主动脉、皮下结缔组织作间皮、内皮及成纤维细胞的培养。取 3类细胞第 3代融合后 4 8h的无血清培养液 ,放射免疫法测定 6 酮 PGF1α(前列环素的代谢产物 )的含量 ,发色底物法测定纤溶酶原激活物活性。结果　间皮细胞培养液中6 酮 PGF1α浓度显著高于成纤维细胞及内皮细胞 ,纤溶酶原激活物活性也高于成纤维细胞 ,但与内皮细胞相比差异无显著性。结论　培养状态下的间皮细胞具有与内皮细胞相似的功能特点 ,可能成为血管假体内腔铺被层的理想材料。更多还原

【Abstract】 Objective: To determine anticoagulant and fibrinolytic activity of cultured mesothelial cells, and provide a basis for selection of artificial vascular prostheses coating cells. Methods: The greater omenta, aortae and subcutanenous connective tissue of SD rats have been taken for mesothelial cells, endothelial cells and fibroblasts culture. The 6-keto-PGF 1α (the metabolite of prostacyclin) concentrations in medium were measured by rodioimmunoassay. Tissue plasminogen activator (t-PA) activity was detected by chromogenic assay. Results: The average concentration of the 6-keto-PGF 1α in mesothelial cells culture was higher than that in endothelial cells and fibroblasts culture, the t-PA activity in mesothelial cells culture was also higher than in fibroblasts, but there was no significant difference between mesothelial cells and endothelial cells. Conclusion: Mesothelial cells have similar functions comparing with endothelial cells. It may be an ideal coating lining for artificial vascular implants.更多还原