【摘要】 目的　研究瑞香狼毒甲醇提取物的抗瘤机理。方法　用不同剂量的瑞香狼毒甲醇提取物 (简称醇提物 ) ,观察其体内对荷瘤鼠和所荷肿瘤生长的作用 ;MTT法研究其体内对荷瘤鼠脾细胞转化及NK活性 ,体外对正常鼠脾细胞生长、转化和NK活性以及对 3株肿瘤细胞增殖的影响 ;流式细胞术和透射电镜检测其体外对K5 6 2的细胞周期和凋亡及p5 3、bcl 2和c myc基因表达的影响 ;台盼蓝拒染法检测其体外对K5 6 2增殖曲线的影响。结果　体内 ,每天 5 μg kg醇提物可抑制肿瘤生长 ,提高荷瘤鼠免疫功能。体外 ,0 .1～ 10 μg ml醇提物可刺激脾细胞增殖和协同最适量和亚适量ConA刺激脾细胞转化 ,0 .1、1μg ml醇提物可提高脾细胞NK杀伤活性 ,0 .1～ 10 μg ml醇提物对K5 6 2、S180和YAC 1的增殖均有抑制作用 ,K5 6 2最为敏感。 0 .5 μg ml醇提物作用的K5 6 2 ,处于G0 G1 期的细胞和凋亡细胞显著增加 ,p5 3基因表达显著增加 ,bcl 2和c myc基因表达显著下降。K5 6 2在 0 .5 μg ml醇提物作用2 4h后洗去或不洗去培养 9d ,细胞虽仍存活 ,但密度却不增加。结论　醇提物在一定剂量范围内可抑制肿瘤细胞生长 ,提高荷瘤鼠免疫功能。抗瘤机理与其可刺激脾细胞增殖 ,协同ConA刺激脾细胞转化和提高脾细胞NK杀伤活性及阻滞肿瘤细胞周期 ,?更多还原

【Abstract】 Objective To study the anti-tumor mechanisms of the methanol extract of Stellera chamaejasme L .（meSCL）. Methods Using different doses of meSCL, the effects of them on the S180-bearing mice and tumor growth were observed in vivo , on the transformation and NK activity of S180-bearing mouse splenocytes in vivo , the growth, transformation and NK activity of normal mouse splenocytes and the growth of three kinds of tumor lines were assayed in vitro by MTT method, on the cytocycle, apoptosis and gene expressions of p53, bcl-2 and c-myc of K562 were investigated by FACS and transmission electron microscopy and on the effect growth curve of K562 was checked by the trypan blue staining-rejecting method. Results Per day 5μg/kg meSCL could inhibit the tumor growth and improve the immune function of S180-bearing mice. In vitro , 0.1～10μg/ml meSCL could stimulate normal murine splenocytes to proliferate and to transform in coordination with the optimal and suboptimal doses of ConA and enhance splenocyte NK activity. meSCL had proliferation-inhibitory effect on K562, S180 and YAC-1 in vitro and K562 cells were the most sensitive ones. The cells of apoptosis and at G₀/G₁ phase of K562 incubating with meSCL were remarkably increased, the p53 gene expression was substantially increased and the gene expressions of bcl-2 and c-myc were significantly decreased. In the period of culturing K562 cells with 0.5μg/ml meSCL for nine days, the tumor cells were all alive but the cell density did not increase. Conclusion A certain dose of meSCL can inhibit the tumor growth and improve the immune function of tumor-bearing mice. The anti-tumor mechanisms of meSCL could be explained by stimulation of the proliferation of splenocyte alone or in coordination with ConA and increase the activity of NK cells and it can hinder the cell cycle of tumor cells, inhibit their fission and proliferation, and promote their apoptosis.更多还原