【摘要】 目的　研究K ras基因突变和CA19 9联合检测在胰腺癌诊断中的作用。　方法　选择同期的 15例胰腺癌和 33非胰腺癌病人 ,同时行外周血K ras基因突变和CA19 9的检测 ,利用统计学方法进行数据分析。　结果　外周血K ras基因突变和CA19 9联合检测诊断胰腺癌的敏感性、特异性分别为 6 6 6 7%和 97% ,研究组和对照组之间差异具有显著性意义 (P =0 0 0 1)。　结论　外周血K ras基因突变和CA19 9的联合检测显著提高了胰腺癌诊断的特异性 ,弥补了单一K ras基因突变和CA19 9检测的不足 ,可用于胰腺癌的辅助诊断。更多还原

【Abstract】 Objective To confirm the effect of combined detection of K-ras gene mutation and CA19-9 level in plasma in the diagnosis of pancreatic cancinoma. Methods In 33 patients without pancreatic carcinoma and 15 patients with pancreatic carcinoma, the value of K-ras mutation and CA19-9 level were examined by enriched polymerase chain reaction (PCR) restriction fragment length polymorphism(RFLP) and radioimmunoassay respectively. The data were analysed with χ 2 test, Fisher′s exact test and Kruekal-wall′s test. All patients were confimed pathologically. Results Eleven of the 15 patients with pancreatic carcinoma and 2 of 10 patients with chronic pancreatitis were found K-ras mutation. CA19-9 level in patients with pancreatic cancer was significantly higher than that in those with benign disease but with a low specificity. The sensitivity, specificity of combined detection of K-ras gene mutation and CA19-9 level in the plasma of patients with pancreatic cancer were 66.67% and 97%. There was a significant difference between patients with pancreatic cancer and those without pancreatic cancer. Pathological examination showed atypical hyperplasia in one patient with chronic pancreatitis who had K-ras mutation and higher CA19-9 level. Conclusions Combined detection of K-ras gene mutation and CA19-9 can be used for adjuvant diagnosis of pancreatic cancer. It is more specific than detection of single K-ras gene mutation or CA19-9 level in the plasma. The method is complementary to single detection of K-ras gene mutation or CA19-9 level because it decreases the rate of false-positive diagnosis.更多还原