【摘要】 目的 探讨结缔组织生长因子(CTGF)在肾间质纤维化中的表达及其在依那普利、黄芪当归合剂的肾脏保护作用中的意义。方法 采用单侧输尿管结扎致肾间质纤维化大鼠模型。将大鼠随机分为4组:假手术组、模型组、黄芪当归组、依那普利组。术后第9天处死各组大鼠,经免疫组织化学(组化)、Western蛋白印迹分析方法观察各组CTGF的表达部位及蛋白表达水平。用免疫组化半定量检测各组肾间质的α-平滑肌肌动蛋白(α-SMA)的表达。用天狼星红染色半定量检测以Ⅰ型胶原为主的肾间质胶原成份。Masson染色评定各组肾小管间质损害程度。结果 模型组CTGF、α-SMA、Ⅰ型胶原的表达及肾小管间质损伤指数明显高于假手术组(P<0.01),而黄芪当归组及依那普利组明显低于模型组(P<0.01)。两治疗组间比较,除结缔组织生长因子外(P<0.01),其余指标无显著性差异(P>0.05)。各项指标作相关分析,CTGF与肾小管间质损伤指数(r=0.788,P<0.01)、α-SMA(r=0.940,P<0.01)、Ⅰ型胶原(r=0.820,P<0.01)为正相关关系。结论 黄氏当归合剂及依那普利可能通过下调CTGF而减轻肾间质纤维化。更多还原

【Abstract】 Objective To investigate the expression of CTGF in rats with renal tubulointerstitial fibrosis and the effects of enalapril and Astragalus-Angelica mixture(A&A) treatment on CTGF protein production. Methods Unilateral ureteral obstruction (UUO) rat animal models were used, and the expression of CTGF,α-SMA, collagen I in the obstructed kidneys was assessed by Western blot, immunohistochemistry and Sirius red staining methods after nine days respectively. At the same time, the degree of tubulointerstitial damage was scored. Results The levels of CTGF, α-SMA, collagen I protein in the UUO group increased remarkably comparing with the sham-operated group (P < 0.01) and paralleled with the degree of tubulointerstitial damage. In comparison with the model group, the levels of CTGF, and expression of α-SMA, collagen I were significantly reduced by both enalapril and A&A treatment. The protein level of CTGF had positive correlation with the index of tubulointerstitial damage ( r = 0. 788, P < 0. 01), α-SMA ( r = 0. 940, P < 0. 01), and collagen I ( r = 0. 820, P < 0. 01). Conclusion Enalapril and A&A may suppress fibrosis development, at least in part via down-regulating CTGF expression.更多还原