【摘要】 目的　探讨人肝癌组织中辅助性T细胞亚群 (Th1/Th2 )细胞因子表达模式。方法　收集我科手术切除肝癌组织标本 15例 ,肝良性疾病或肝硬化门静脉高压手术患者肝组织标本 15例作为对照 ,β 肌动蛋白 (β actin)作为参照 ,采用半定量逆转录 聚合酶联反应 (RT PCR)方法检测人肝癌组织中γ 干扰素 (IFN γ)和白细胞介素 4(IL 4)mRNA的表达。结果　人肝癌组织中IFN γmRNA表达 (相对数 :1.18± 0 .3 1)比对照组肝组织中IFN γmRNA表达弱 (相对数 :1.86±0 .5 0 ) ,而IL 4mRNA表达 (相对数 :1.73± 0 .5 1)比对照组强 (相对数 :0 .94± 0 .41,P <0 .0 5 )。结论　人肝癌组织中Th1类细胞因子表达弱 ,Th2类细胞因子表达强 ,呈现Th2细胞因子表达模式 ,肝癌组织局部微环境处于免疫抑制状态 ,肿瘤细胞易发生免疫逃逸更多还原

【Abstract】 Objective To study the expression of Th1/Th2 type cytokine in human liver cancer tissue.Methods 15fresh liver cancer tissue samples and 15 liver tissue specimens from benign liver disease and cirrhosis with hypersplenism were obtained from surgical resections at our liver surgical centre.The mRNA expression of Th1 type and Th2 type cytokine in fresh human liver cancer tissue and liver tissue of the control group was detected by RT PCR using β actin as a positive control.Results The IFN γ mRNA expression of human liver cancer tissue (relative number 1.18±0.31) was significantly lower than that of control group liver tissue (relative number 1.86±0.50),but the IL4 mRNA expression of human lier cancer tissue (relative number 1.73±0.51) was significantly higher than that of control group liver tissue (relative number 0.94±0.41) ( P <0.05).Conclusion Human liver cancer tissue produces Th2 type cytokine expression pattern.Liver cancer microenviroment develops immunosuppression,which may be related to evasion of tumor cells from immunosurveillance.更多还原