【摘要】 目的　观察干扰素 β 1b(IFNβ 1b)在体外对多发性硬化 (MS)患者趋化因子mRNA表达的影响。方法　取MS患者外周血 ,分离单个核细胞 (MNC) ,以其他非炎性神经系统疾病 (OND)及健康人 (HC)为对照组。MNC在完全培养基中分别与自身抗原髓鞘碱性蛋白 (MBP)、对照抗原AChR及不加抗原组 ,加或不加药物IFNβ 1b共同培养 ,3d后收集细胞 ,涂片 ,用地高辛标记的寡核苷酸探针进行原位杂交 (ISH) ,检测C C趋化因子单核细胞炎性蛋白 1α/ β(MIP 1α/ β)、单核细胞趋化蛋白 1(MCP 1 )和正常T细胞表达及分泌的调节活化因子 (RANTES)mRNA的表达。结果　MBP刺激的MIP 1α及自发产生的MIP 1αmRNA均受到IFN β 1b的抑制 ,但差异无显著意义 (P >0 0 5)。RANTESmRNA的表达受到IFNβ 1b的抑制 ,在MBP诱导下无药物处理时为 30 2± 1 5 0 (细胞数 / 1 0 5,下同 ) ,有药物处理为 1 1 1± 5 3 ,差异有显著意义 (P <0 0 1 ) ;在无抗原诱导下无药物处理时为 1 8 5± 3 3 ,有药物处理为 5 1± 3 2 ,差异亦有显著意义 (P <0 0 1 )。IFNβ 1b在 1 0U/ml浓度下 ,可对MBP刺激的MCP 1mRNA的表达产生抑制作用 (分别为 1 58 4± 1 0 4 3和 63 2± 36 9,差异有显著性意义 ,P <0 0 1 ) ,而对自发产生的MCP 1mRNA作用不明显 ;对MBP刺激更多还原

【Abstract】 Objective To define the in vitro effects of IFNβ 1b on the chemokine mRNA expression in Mononuclear cells(MNC)of patients with multiple sclerosis (MS) Methods MNCs were prepared from blood of patients with MS,other neurological diseases (OND) and healthy control (HC) The expression of MIP 1α/β mRNA,MCP 1 mRNA and RANTES mRNA were examined in situ hybridization after 3 days of incubation with MBP,AChR and IFN β 1b Results Both the MIP 1α simulated by MBP and the self produced MIP 1α mRNA were suppressed by IFN β 1b,but there showed no significant difference( P >0 05) There existed marked distinction in the expression of RANTES mRNA repressed by IFN β 1b Under the inducement of MBP,it was 30 2±15 0 (/10 5,same below) without IFN β 1b,while 11 1±5 3 after treatment( P <0 01) Without MBP,it was 18 5±3 3 without treatment,compared notably with 5 1±3 2 under treatment of IFNβ 1b( P <0 01) At a concentration of 10 U/ml,the IFN β 1b might inhibit the expression of MCP 1 mRNA incited by MBP (158 4±104 3 vs 63 2±36 9 with a notable difference, P <0 01),but has no patent effect on the self produced MCP 1 mRNA,the MIP 1β mRNA produced spontaneously or stimulated by MBP Conclusions The expressions of RANTES and MCP 1 mRNA could be inhibited by IFN β 1b in vitro These findings should be potentially relevant to the therapeutic mechanism of IFNβ 1b in MS更多还原