【摘要】 目的　分析肾综合征出血热山东分离株鲁宁 84刘株的分子特征。方法　应用逆转录聚合酶链反应 (RT PCR)扩增鲁宁 84刘株M和S片段全基因 ,克隆于T载体 ,纯化后测定序列 ,应用DNASTAR软件比较分析。结果　鲁宁 84刘株 (JNL株 )的M片段的全基因序列共 36 15个核苷酸 ,编码 1135个氨基酸 ,S片段的全基因序列共 16 98个核苷酸 ,编码 4 2 9个氨基酸 ,为HTN型毒株。JNL株与HTN型毒株M和S全基因序列差异分别高达 2 0 0 %～ 2 0 6 %和 15 5 %～ 16 0 % ,基于核苷酸序列的种系发生分析结果也可以看到 ,鲁宁 84刘株不同于国内外其他的分离株 ,处于独立的分支。结论　山东地区鲁宁 84刘株流行株是与国内外HTN型毒株不同的新的HTN亚型病毒。更多还原

【Abstract】 Objective To understnad the molecular epidemiologic characteristics of hantavirus Shandong isolate JNL virus strain Methods The complete M and S gene of the JNL virus isolated from Shandong Province was amplified by RT\|PCR,and the purified PCR product was cloned into T vector for sequencing Results The results revealed that the JNL M segment was 3 615 bp in length,encoding 1 135 amino acids,and the S segment was 1 698 bp encoding 429 amino acids,JNL belongs to HTN virus The comparison of homology with HTN and SEO types showed that the difference of M and S complete sequences between JNL and all other HTN virus strains reached 20\^0% 20\^6% and 15\^5% 16 0% respectively Phylogenetic tree also showed that the position of JNL is located at a different clade Conclusion HTN virus Shandong local isolate JNL strain is a new specific HTN subtype virus更多还原