【摘要】 目的　探讨氯化镧对创伤组织中成纤维细胞凋亡的影响及其防治瘢痕的可能性。　方法　选用 5 0只成年SD大鼠 ,在其背部近四肢关节处各制作 1个切口。其中 1个切口不作任何处理 ,作为空白对照组 ;其余切口两侧皮下各注射三蒸水、5 0mmol/L氯化镧以及 0 .2mg/ml的抗转化型生长因子β1抗体 (注射量均为 0 .2 5ml),相应设为模拟对照组、氯化镧组和抗体组。用药后 14、2 8d,采用流式细胞仪 (FCM)和原位末端酶标记 (TUNEL)法 ,检测伤口组织标本中成纤维细胞凋亡情况及细胞内Ca2 + 浓度的变化。　结果　与两对照组比较 ,用药 14d后 ,氯化镧组及抗体组伤口成纤维细胞凋亡明显增强 (P <0.0 5～ 0.0 1),前者细胞内Ca2 + 浓度明显增高 (P <0.0 1)。　结论　提示氯化镧具有一定的防治瘢痕作用更多还原

【Abstract】 Objective To investigate the effects of lanthanum chloride on the apoptosis of fibroblasts in trauma tissue. Methods Fifty adult female SD rats were used and linear incisions were made on the back near the joints of extremities of the rats. One of the cuts receiving no treatment was designated as blank control (C). 0.25 ml of distilled water, lanthanum chloride (50 mmol/L) and the antibody of (TGFβ 1)transforming growth factor β 1 (0.2 mg/ml) were respectively injected into the both sides of the other three wounds subcutaneously and the wounds were divided into simulating control (SC), lanthanum chloride(LC) and antibody (A) groups. The fibroblast apoptosis in the wound tissue samples and the change in intracellular calcium concentration (Ca 2+) were determined by flow cytometry (FCM) and TUNEL methods on the 14th and 28th day after the injection. Results Apoptosis of fibroblasts was enhanced significantly after 14 days of injection in LC and A groups compared with that in C and SC groups (P < 0.05～0.01). Furthermore, intracellular Ca 2+ was increased evidently in LC group (P< 0.01). Conclusion It is indicated that lanthanum chloride might be effective in preventing scar development.更多还原