【摘要】 目的　研究改进肝细胞腹腔移植的方法。方法　用胶原凝胶包埋大鼠肝细胞 ,并在体外培养 ,测定培养液中总蛋白 (TP)和尿素氮 (BUN)水平。将胶原培养液与鼠肝细胞混合后注入大鼠腹腔 ,混合物在腹腔内形成凝胶并包埋肝细胞。用含酶的培养液将受者鼠腹腔内的移植肝细胞洗出 ,再置于无葡萄糖培养液中培养 ,测定培养液中葡萄糖含量。对照组处理过程与实验组相同 ,但不用胶原。结果　胶原凝胶包埋肝细胞组的TP、BUN和葡萄糖含量均明显高于对照组 (P <0 .0 1)。胶原培养液与肝细胞混合物在注入腹腔 2 4h后变为凝胶。结论　胶原凝胶可促进体外培养或腹腔移植的肝细胞生长 ;胶原培养液腹腔注射和腹腔内胶原凝胶包埋细胞法 ,使肝细胞的腹腔移植更简便、安全、实用。更多还原

【Abstract】 Objective To improve the method of abdominal transplantation of hepatocytes. Methods The rat hepatic cells were embedded in collagen gel and cultured in vitro. The total protein (TP) and BUN levels in the nutrient solution were measured. The mixture of collagen nutrient solution and the hepatocytes was injected into abdominal cavity of rat recipients and then turned to gel there, so the hepatocytes were embedded in the gel. The hepatocytes in the abdominal cavity were rinsed out by the nutrient solution containing collagenase and were cultured in vitro in the nutrient solution without glucose (Glu). The Glu levels in the nutrient solution were measured. The control groups underwent the same process as the experimental groups except collagen. Results The TP, BUN and Glu levels in the experimental groups (hepatocytes embedded in collagen gel) were significantly higher than those in the control groups ( P < 0.01 ). Twenty-four h after injection, the mixture of collagen nutrient solution and hepatocytes turned to gel in the abdominal cavity. Conclusions The mixture of collagen nutrient solution and the hepatocytes could be injected into abdominal cavity of rats and turned to gel there. Gel could improve the growth of the hepatocytes transplanted in abdominal cavity as well as cultured in vitro.The abdominal injection of the collagen nutrient solution could make the abdominal transplantation of hepatocytes easier, safer and more practicable.更多还原