【摘要】 目的研究抑癌基因p15、p16对人胆管癌细胞的生长抑制作用。方法将抑癌基因p15、p16的cDNA构建到真核表达的质粒载体pcDNA3 neo中形成p15真核表达质粒pcDNA3p15及p16真核表达质粒pcDNA3p16。通过脂质体法将重组质粒pcDNA3p15、pcDNA3p16质粒分别转染人胆管癌细胞系QBC939,获得稳定高表达p15或p16的人胆管癌细胞模型。用MTT法测定细胞生长曲线 ,流式细胞光度术测定细胞生长周期。结果p15、p16高表达的人胆管癌细胞QBC939的增殖受到抑制 ,p15、p16均明显阻遏细胞由G1期向S期的转换。结论抑癌基因p15、p16均参与了细胞周期的阻抑作用。更多还原

【Abstract】 ObjectiveTo study the effect of anti-oncogene p15 and p16 on the proliferation of human cholangiocarcinoma cell line.MethodsThe cDNA of anti-oncogene p15 and p16 was constructed into pcDNA3-neo plasmid carrier. The human cholangiocarcinoma cell line QBC939 were transfected with the recombinants pcDNA3p15 and pcDNA3p16 using lipofectin, respectively. The expression products were analyzed by Western blot. Cell viability and death were measured with MTT assay. Cell cycle was determined by flow cytophotometry and the formation of cell clone was detected. Results The growth of QBC939 cells was inhibited. The flow cytophotometry verified p15 and p16 induced QBC939 cell G1 blockade. Conclusion Anti-oncogene p15 and p16 together lead to the inhibition of cell cycle.更多还原