【摘要】 采用 RP- HPL C法在 335 nm处测定血浆中灯盏乙素的浓度。双周期自身交叉设计 ,beagle犬随机静注 90 mg或口服 1.8g灯盏乙素 ,6 d后交叉试验。血浆中灯盏乙素的定量限为 0 .0 2 5 μg/ ml;方法回收率大于 93.2 % ,日内、日间 RSD小于 8.31% ;灯盏乙素血浆样品常温下不稳定 ;静注给药药 -时曲线符合三室模型。经剂量校正 ,口服给药的绝对生物利用度为 (0 .4 0± 0 .19) %。灯盏花素静注给药体内消除迅速 ,口服给药几乎不吸收更多还原

【Abstract】 Scutellarin in plasma was determined by RP HPLC with ultraviolet detection at 335 nm. According to a randomized two period crossover design, the beagle dogs were iv 90 mg and oral admini strated of 1800 mg of scutellarin, respectively, with a 6 day washout. The limit of quantification for scutellarin was 0.025 μg/ml, the intra and inter day RSD s were less than 8.31%, and the average reco very was above 93.2%. It was found that scutellarin in plasma was unstable under room temperature. The pharmacokinetics behavior of scutellarin iv could be described by a three compartment model. The absolute bioavailability of scutellarin for oral administration was (0.40±0.19)%. The elimination of iv breviscapine in dogs was fast, but it was almost not absorbed for oral administration.更多还原