【摘要】 目的　探讨凝血酶原 (PT)分子中γ 羧基谷氨酸 (Gla)在抑制草酸钙结晶形成中的作用。　方法　采用加入不同浓度的吗啉和甲醛 (1∶10 0 0 0 )或 (1∶10 0 ) ,体外化学修饰PT ,将其分子内8个或 2个γ 羧基谷氨酸残基修饰转变成γ 亚甲基谷氨酸残基 (γ MGlu) ,草酸钙种晶抑制试验测定化学修饰后PT对草酸钙结晶抑制作用的变化。　结果　PT蛋白转变为 2γ MGlu的PT后对草酸钙结晶的抑制活性从正常对照的 14 .2降低至 12 .8(10 % ) ;PT蛋白转变为 8γ MGlu后其抑制活性降低至 5 .0 (6 5 % )。　结论　凝血酶原分子内γ 羧基谷氨酸 (Gla)在抑制草酸钙结晶形成中具有重要作用。更多还原

【Abstract】 Objective To study and evaluate the inhibitory role of γ-carboxyglutamic acid(Gla)in the Gla domain of prothrombin in the formation of calcium oxalate crystals. Methods The addition of morpholine and formaldehyde to the solution of prothrombin caused the conversion of 8 Gla to γ-methyleneglutamic acid (8γ-MGlu)or 2 Gla to γ-methyleneglutamic acid(2γ-MGlu) ,the inhibitory activity of which to calcium oxalate crystallization was detected with seeded crystallization technique. Results The conversion of Gla to γ-MGlu leads to the decreased inhibitory activity of prothrombin to calcium oxalate crystallization,the inhibitory index being decreased from normal 14.2 to 12.8 of 2γ-MGlu or 5.0 of 8 γ-MGlu prothrombin respectively. Conclusions Gla in prothrombin may play an important role to inhibit the formation of calcium oxalate calculi.更多还原