【摘要】 目的　研究内源性转化生长因子 β1(TGFβ1)对膀胱癌细胞增殖的影响及机制。　方法　构建携TGFβ1反义RNA的复制缺陷型逆转录病毒载体pRevTβ AS ,转染膀胱癌EJ细胞株 ,观察抑制内源性TGFβ1表达对EJ细胞体外增殖的影响 ;流式细胞仪分析转染后细胞周期时相分布的改变。　结果　将TGFβ1反义RNA导入EJ细胞并有效表达 ;抑制TGFβ1表达可以降低靶细胞的生长速度和克隆形成率 ,使细胞增殖指数下降 12 % ;反义RNA转染的EJ细胞G0 和G1期细胞占 65 .7% ,高于对照组细胞 ,S期细胞占 2 0 .5 % ,低于对照组细胞。　结论　内源性TGFβ1通过诱导膀胱癌细胞G1→S期转换 ,促进肿瘤细胞体外增殖更多还原

【Abstract】 Objective To investigate the influence of endogenous transforming growth factor β 1 (TGFβ 1) on the cell cycle regulation and proliferation of bladder cancer. Methods A constructed replication defective retroviral vector pRevTβ AS,which carried antisense RNA of TGFβ 1,was transfected to a bladder cancer cell line EJ.The proliferation and clone formation of transferred cells were observed in vitro,and the alteration of cell cycle was also detected by flow cytometric analysis. Results TGFβ 1 antisense RNA was transferred into EJ cell and expressed efficiently.After the inhibition of target gene expression in EJ cells,the reduced growth and clone formation rates were demonstrated,and the proliferative indexes were decreased by 12%.The ratios of G 0 and G 1 stage cells to the antisense RNA transfected EJ cells were increased,simultaneously,the ratio of S stage cells to the antisense RNA transfected EJ cells ratios were decreased,compared with the control group. Conclusions The proliferative mechanism of endogenous TGFβ 1 in bladder cancer cells is to stimulate the G 1 to S stage transition.更多还原