【摘要】 目的　了解深圳市一种新的单链DNA病毒 (SENV)的感染流行状况。方法　以SENV读码框架 1区 (ORF1)核苷酸序列设计引物建立巢式聚合酶链反应 (nPCR)方法。采用多重nPCR对6 0 1份来自不同人群血标本进行SENV DNA(D和H亚型 )检测 ,并对所分离的病毒部分基因进行克隆后测序分析。结果　在乙型肝炎患者、丙型肝炎患者、血液透析患者和吸毒人群SENVDNA阳性率分别为 2 7.8%、2 2 .2 %、2 6 .9%和 39.3% ;在献血人群中 ,体检不合格献血者和丙氨酸转氨酶 (ALT)异常升高献血者SENV阳性率分别为 2 8.1%和 31.3% ,均明显高于体检合格的献血者人群 (15 .1% )的感染率(χ2 =8.2 9,P <0 .0 1和χ2 =6 .0 3,P <0 .0 1)。 6例来自不同人群的SENV D亚型分离株部分核苷酸序列与标准株变异 <6 .8% ;4例来自不同人群SENV H亚型分离株部分核苷酸序列与标准株核苷酸最大变异为 13.5 %。结论　在深圳市高危人群 (献血和吸毒人群及肝炎患者等 )中SENV感染均广泛存在更多还原

【Abstract】 Objective To investigate the prevalence of newly identified single-chain DNA virus (SENV) infection in Shenzhen. Methods Nested polymerase chain reaction (nPCR) was established using primers from ORF1 region of SENV genome. Six hundred and one sera samples from different populations were detected for SENV DNA (D and H subtype) by nPCR. Products of PCR were cloned into T- vector and sequenced. Results The positive rates of SENV DNA in different populations were as followes: 27.8 % in patients with hepatitis B, 22.2 % in patients with hepatitis C, 26.9 % in hemodialysis patients and 39.3 % in IDUs. Among blood donors, the positive rates of SENV DNA were 28.1 % in unqualified blood donors, 31.3 % in blood donors with an elevated ALT levels and 15.1 % in qualified blood donors. The infection rates of SENV in unqualified blood donors and blood donors with an elevated ALT levels were obviously higher than in qualified blood donors ( χ 2= 8.29 , P < 0.01 and χ 2= 6.03 , P < 0.01 ). There was a 6.8 % difference of nucleotide between SENV- D standard subtype and 6 isolates with 13.5 % difference of nucleotide between SENV- H standard subtype and 4 isolates from Shenzhen. Conclusion Results suggested that SENV infection was common in high- risk groups in Shenzhen.更多还原