【摘要】 [目的]分析凋亡信号蛋白(FADD、TRADD、FasL、Fas和NFκB)在人类肝癌组织中的表达及其临床意义。[方法]采用Westernblot法检测肝癌组织中上述5种凋亡信号蛋白的表达情况。[结果]在35例HCC标本中,FADD、TRADD、FasL、Fas和NFκB蛋白表达分别为37.14%(13/35)、68.57%(24/35)、68.57%(24/35)、60%(21/35)和77.14%(27/35)。其中,Fas蛋白表达随肿瘤增大而下降,同肿瘤大小之间差异有显著性(P<0.05)。FADD和Fas两蛋白表达随肿瘤恶性程度增高,而明显下降,与病理分级间差异有显著性(P<0.01,P<0.05)。TRADD和Fas蛋白表达同甲胎蛋白水平呈正相关(P<0.01)。各蛋白表达同肿瘤转移无关(P>0.05)。[结论]肝癌细胞可通过下调引起凋亡的信号蛋白的表达,如Fas,FADD;和上调引起存活信号蛋白的表达,如TRADD,NFκB等机制的协同作用,来逃避凋亡的发生,从而达到维持自身稳定的目的,这对肝癌的发展起重要作用。更多还原

【Abstract】 To investigate the expression of apoptosic signal proteins (FADD,TRADD,FasL,Fas and NFκB) in human hepa-tocelluar carcinoma (HCC) tissue and its correlation with clinical outcome. Western blot was used to detect the level of five kinds of apoptosic signal proteins in HCC tissue. The expression rates with FADD,TRADD,FasL,Fas and NFκB proteins were 37.1% (13/35),68.57% (24/35),68.57% (24/35),60% (21/35) and 77.14% (27/35) respectively in HCC.The difference between expression of Fas protein and tumor volumn was significant (P<0.05).Positive expressions of FADD and Fas protein increased with the increase of tu-mor grade (P<0.01,P<0.05).The expressions of TRADD and Fas positively correlate with the level of AFP (P<0.01).The expression of apoptosic signal proteins did not correlate with tumor metastasis (P>0.05).[Conclusion] HCC cell escapes apoptosis and maintains stability of itself by the cooperative function with down regulation of the expressions of the signal proteins caused apoptosis such as Fas and FADD and with up regulation of those leading to survival,such as TRADD and NFκB.更多还原