【摘要】 目的　探讨苯并 [a]芘 (BaP)诱导猪主动脉内皮细胞细胞色素P4 5 0 1A1(CYP1A1)表达及活性的影响。方法　离体培养猪主动脉内皮细胞 ,不同浓度BaP( 0、0 .5、1.0、5 .0、10 .0 μmol L)染毒 2 4h ,分别以Western blot法和免疫组化方法检测不同浓度BaP诱导内皮细胞合成CYP1A1的影响 ,同时还探讨了乙氧基异吩口恶唑 o 去乙氧基酶 (EROD)活力的变化规律。结果　Western blot法未能检测出对照组CYP1A1的表达 ,而各染毒组均检出了CYP1A1的表达 ;免疫组化结果显示染毒组仅在部分内皮细胞呈阳性反应 ;EROD活力诱导高峰的BaP浓度为 0 .5～ 1.0 μmol L。结论　BaP可诱导部分猪主动脉内皮细胞合成CYP1A1;EROD活力诱导高峰的BaP浓度为 0 .5～ 1.0 μmol L。更多还原

【Abstract】 Objective To explore the effect of benzo(a)pyrene(BaP) on the expression and the activities of cytochrome P450 1A1(CYP1A1) of porcine aortic endothelial cells. Methods Porcine aortic endothelial cells were cultured in vitro,and treated with different concentrations of BaP(0,0.5,1.0,5.0,10.0 μmol/L) for 24 hours,CYP1A1 expression was determined by Western blot and immunohistochemistry.At the same time,the ethoxyresorufin o deethylase(EROD) activities were measured by spectrofluorometer. Results By Western blot,the expression of CYP1A1 of control cells was not found,but the expression of CYP1A1 of cells treated with BaP was found;By immunohistochemistry,only part of endothelial cells treated with BaP had positive expression of CYP1A1.The peak activities of EROD induced by BaP was at the concentration of 0.5～1.0 μmol/L. Conclusion BaP could induce part of endothelial cells to synthesize CYP1A1.BaP of 0.5～1.0 μmol/L could induce peak activities of EROD.更多还原