【摘要】 目的　研究极低频电磁场暴露对小鼠脑和肝细胞凋亡及细胞周期的影响。方法　小鼠经 5 0Hz、0 .2mT或 5 0Hz、6 .0mT电磁场暴露 2周 ,采用TUNEL法观察凋亡细胞 ,采用流式细胞术检测细胞凋亡率和细胞周期。结果　 0 .2mT和 6 .0mT电磁场暴露后 ,脑细胞凋亡率分别为 ( 5 .6 0±1.4 7) %和 ( 4 .73± 0 .4 8) % ,与对照组 [( 2 .90± 0 .75 ) % ]比较 ,差异有显著性 (P <0 .0 5 ) ;肝细胞凋亡率分别为 ( 4 .19± 2 .0 8) %和 ( 3.38± 0 .6 5 ) % ,与对照组 [( 1.84± 0 .76 ) % ]比较 ,差异亦有显著性 (P <0 .0 5 )。脑细胞G0 G1期细胞百分率分别为 ( 80 .2 1± 1.6 8) %和 ( 79.5 4± 0 .5 6 ) % ,肝细胞G0 G1期细胞百分率分别为 ( 79.4 2± 1.80 ) %和 ( 80 .4 7± 1.79) % ,与对照组 [分别为 ( 76 .85± 0 .83) %、( 73.36±3.10 ) % ]比较 ,差异均有显著性 (P <0 .0 5 )。与此同时 ,S期和G2 +M期的细胞百分率明显下降。结论　极低频电磁场暴露可能诱发小鼠脑和肝细胞周期改变 ,并可能进一步导致细胞凋亡。更多还原

【Abstract】 Objective To study the effects of extremely low frequency electromagnectic fields(ELF EMFs) on apoptosis and cell cycle of mouse brain and liver cells. Methods Mice were exposed to 50 Hz,0.2 mT or 6.0 mT electromagnetic fields for 2 weeks.TUNEL and flow cytometric methods were used to analyze apoptosis and cell cycle of brain and liver cells. Results After exposure to 0.2 mT and 6.0 mT ELF EMFs for 2 weeks,apoptosis rates of brain cells[(5.60±1.47)% and (4.73±0.48)% respectively] were higher than that of control[(2.90±0.75)%],and apoptosis rates of liver cells[(4.19±2.08)% and (3.38±0.65)% respectively] were higher than that of control[(1.84±0.76)%].G0/G1 cell percentage of brain cells[(80.21±1.68)% and (79.54 ±0.56)% respectively] were higher than that of control[(76.85±0.83)%],and those of liver cells [(79.42 ±1.80)% and (80.47±1.79)% respectively] were higher than that of control[(73.36±3.10)%].The above differences were all statistically significant as P<0.05.At the same time S and G2+M cell percentage of brain and liver cells were significantly decreased. Conclusion Exposure to 50 Hz EMFs may alter cell cycle and induce apoptosis of mouse brain and liver cells.更多还原