【摘要】 目的　研究 2 4 5 0MHz(5mW cm2 )微波 (MW)与 3种化学诱变剂对人淋巴细胞DNA损伤的联合作用。方法　采用彗星试验体外实验检测淋巴细胞经微波和化学诱变剂单独及联合作用后 ,不同培养时间 (0h和 2 1h)的DNA损伤。微波与化学诱变剂联合暴露方式有 3种 :微波辐射后化学诱变剂染毒、微波与化学诱变剂同时暴露、化学诱变剂染毒后微波辐射。 3种化学诱变剂是丝裂霉素C(MMC ,DNA交联剂 )、博来霉素 (BLM ,似X线剂 )、甲基甲烷磺酸酯 (MMS ,烷化剂 )。微波辐射和化学诱变剂暴露时间分别是 2h和 3h。结果　微波组培养 0h和 2 1h ,彗星尾长与对照组比较 ,差异无显著性 (P >0 .0 5 )。培养 2 1h后 ,当MMC浓度为 30 .0 0 μmol L时 ,MW +MMC组、MW MMC组和MMC +MW组的尾长分别为 (18.0 0± 5 .96 )、(2 1.79± 11.4 7)、(2 2 .32± 8.10 ) μm ;当MMC浓度为 3.0 0μmol L时 ,尾长分别为 (8.99± 3.75 )、(12 .4 0± 5 .35 )、(14 .0 0± 5 .38) μm ,明显高于相应的MMC组[(9.4 2± 3.34) μm和 (6 .5 0± 2 .89) μm],差异有显著性 (P <0 .0 1或P <0 .0 5 )。MW +BLM组和MW +MMS组的DNA损伤与相应的BLM组和MMS组比较 ,差异无显著性 (P >0 .0 5 )。结论　 2 4 5 0MHz(5mW cm2 )微波辐射 2h未观察到明显的人淋巴细胞更多还原

【Abstract】 Objective To study the combined damage-effects of low-intensity 2 450 MHz microwave(MW) with three chemical mutagens on human lymphocyte DNA. Methods DNA damage of lymphocytes exposed to microwave and(or) with chemical mutagens were observed at different incubation time(0 h or 21 h) with comet assay in vitro.Three combination-exposure ways of MW with chemicals were used:MW irradiation before chemical exposures,simultaneously exposed to MW and chemicals and MW irradiation after chemical exposures.The three chemical mutagens were mitomycin C(MMC,DNA crosslinker),bleomycin(BLM,radiometric agent),methyl methanesulfonate(MMS,alkylating agent).The exposure time of MW and chemical mutagens were 2 h and 3 h respectively. Results The differences of comet tail length between MW group and control group were not significant when lymphocytes were incubated for 0 h or 21 h( P >0.05).However,when lymphocytes were incubated for 21 h with 30.00 μmol/L of MMC,the comet tail lengths of MW+MMC group,MW-MMC group and MMC+MW group were (18.00±5.96),(21.79±11.47) and (22.32±8.10)μm respectively;while with 3.00 μmol/L of MMC,the comet tail lengths were (8.99±3.75),(12.40±5.35) and (14.00±5.38)μm respectively,which were significantly higher than those of corresponding MMC groups[(9.42±3.34) and (6.50±2.89)μm, P <0.01 or P <0.05].The DNA damage of MW plus BLM groups and MW plus MMS groups were not significantly different from the corresponding BLM and MMS groups( P<0.05) . Conclusion 2 450 MHz MW(5 mW/cm 2)did not induce DNA damage directly,but could enhance the DNA damage effects induced by MMC.The synergistic effects of 2 450 MHz MW with BLM and MMS were not obvious.更多还原