【摘要】 目的　探讨染矽尘小鼠肺组织转化生长因子 β1(TGF β1)基因的表达在矽肺发病中的意义。方法　采用暴露式气管内注入法给小鼠一次性染矽尘 (0 .2g/kg体重 ) ,染尘后第 1、3、5、7、14、2 8天 ,每组各处死 8只小鼠 ,取肺组织 ,用逆转录聚合酶链式反应 (RT PCR)方法测定小鼠肺组织TGF β1基因的表达。结果　染尘后第 3天 ,小鼠肺组织中TGF β1基因表达开始升高 (1.2 0± 0 .15 ) ;第 7天达高峰 (1.74± 0 .19) ,与对照组 (1.0 1± 0 .16 )和染尘第 5天 (1.35± 0 .15 )比较 ,差异均有显著性 (P <0 .0 5 ) ;第 14天开始下降 ,但直到第 2 8天仍高于对照组。染矽尘小鼠肺组织羟脯氨酸含量随染毒后时间的延长 (1～ 2 8d)呈现持续上升的趋势。结论　TGF β1在矽肺早期的发生、发展过程中可能有重要作用。更多还原

【Abstract】 Objective To investigate the gene expression of transforming growth factor-β₁ （TGF-β₁ ） in lung tissues of silica-treated mice. Methods The experimental mice were divided into control and silica group. 0.2 g/kg body weight of silica was injected intratracheally in silica group.Samples of lung tissue were collected 1,3,5,7,14 and 28 d after injection.RT-PCR method was used to analyze the gene expression of TGF-β₁ in lung tissue of silica-treated mice. Results The expression of TGF-β₁ gene in lung tissue elevated from the 3rd day（1.20±0.15） and the peak value was on the 7th day（1.74±0.19）.Then the expression decreased from the 14th to 28th day.But there was still higher than control until the 28th day. Conclusion TGF-β₁ may play an important role in silica-induced pulmonary fibrosis.更多还原