【摘要】 目的　探讨慢性职业应激对行车调度人员糖皮质激素受体 (GR)及免疫功能的影响。方法　采用职业应激量表 ,将接受调查的 112名行车调度人员分为重、中、轻度职业应激组。采集外周静脉血 ,测定血清皮质醇 (GCs)含量、GR水平和CD3、CD4 、CD8等淋巴细胞表面分子阳性细胞的百分率 ,以及血清白细胞介素 2 (IL 2 )水平 ,并对各项指标进行相关分析。结果　与轻度职业应激组血清GCs含量 [(181.0 1± 5 3 .41)ng/ml]相比 ,重、中度职业应激组GCs含量明显增高 [分别为 (2 95 .43±79 .0 6 )、(2 74.34± 70 .0 8)ng/ml],差异有显著性 (P <0 .0 5 ) ;而外周淋巴细胞GR水平明显低于轻度职业应激组 [分别为 (4330 .0± 10 0 1.0 )、(3 971.6± 96 6 .8)、(5 141.3± 10 6 8.5 )位点 /细胞 ],差异亦有显著性 (P <0 .0 5 ) ;重度职业应激组T细胞CD3 表达百分率明显低于轻度职业应激组 [分别为 (5 0 .2 1±10 .30 ) %、(5 6 .87± 15 .36 ) % ],差异有显著性 (P <0 .0 5 ) ;重、中度职业应激组CD4 百分率明显低于轻度职业应激组 [分别为 (2 3 .2 7± 10 .0 1) %、(2 7.0 6± 7.47) %、(33 .31± 7.77) % ];而重、中度职业应激组的CD8百分率则明显高于轻度职业应激组 [分别为 (2 8.16± 6 .47) %、(2 5 .5 4± 6 .70 ) %?更多还原

【Abstract】 Objective To study the effect of chronic occupational stress on the glucocorticoid receptor(GR) and immune function. Methods A cross-sectional study was conducted in 112 railway station dispatchers.Perceived job stress was assessed by means of the Chinese version of the Job Content Questionnaire.The subjects were divided into high,medium and low strain groups according to the job strain score of the questionnaire.The number of GR,percentage of T lymphocyte subpopulations,concentrations of cortisol and interleukin 2(IL-2) in blood were measured. Results The concentrations of serum cortisol in high and medium strain groups[(295.43±79.06) and (274.34±70.08)ng/ml respectively] were higher than that in low strain group[(181.01±53.41)ng/ml,P<0.05].GR binding capacity in both groups(4 330.0±1 001.0,3 971.6±966.8 specific binding/cell respectively) were smaller than that in low strain group (5 141.3±1 068.5 specific binding/cell,P<0.05).The percentage of CD 3 T lymphocyte in high strain group was higher than that in low strain group[(50.21±10.30)% vs (56.87±15.36)%,P<0.05],and CD 4 T lymphocyte in high and medium strain groups were significantly smaller than that in low strain group [(23.27±10.01)%,(27.06±7.47)% vs (33.31±7.77)%,P<0.05].In contrast,the percentage of CD 8 T lymphocytes in high and medium strain groups were significantly higher than that in low strain group [(28.16±6.47)%,(25.54±6.70)% vs (21.91±5.93)%,P<0.05].The levels of serum IL-2 in high and medium groups were smaller than that in low strain group[(0.77±0.05),(0.80±0.07) vs (1.05± 0.12)ng/ml,P<0.05].Correlation analysis showed that serum cortisol level was negatively correlated with CD 8 percentage(r=-0.612,P<0.01). Conclusion Chronic occupational stress may induce rise of glucocorticoid,down-regulation of GR and inhibition on immune function.更多还原