【摘要】 目的　探讨儿童口腔黏膜良性上皮增生性病损与人乳头状瘤病毒 (humanpapillomavirus,HPV)感染及其类型的关系。方法　选取四川大学华西口腔医学院病理科近 10年 30例儿童口腔黏膜良性上皮增生性病损的病例 ,复习其临床病理特征及切片 ,并采用免疫组织化学及原位杂交方法检测HPV共同抗原及其类型。结果　口腔鳞状细胞乳头状瘤 (squamouscellpapilloma ,SCP)2 0例 (6 6 7% ) ,尖锐湿疣 (condylomaacuminatum ,CA) 6例 (2 0 0 % ) ,口腔黏膜局灶性上皮增生 (focalepithelialhyperplasia,FEH) 4例 (13 3% )。HPV检测结果显示 :HPV共同抗原阳性者占 73 3% (2 2 /30 ) ,其中SCP占 75 .0 % (15 /2 0 ) ,6例CAHPV共同抗原均为阳性 ,4例FEH中仅 1例HPV共同抗原为阳性 ;HPV类型以高危型HPV16 /18为主 ,占 77 3% (17/2 2 ) ,其次是HPV6和HPV11。结论儿童口腔黏膜良性上皮增生性病损与HPV感染关系密切 ,病毒类型以高危型HPV16 /18为主 ,其病毒类型是否与成人 (以HPV6、11为主 )不同尚待进一步研究。更多还原

【Abstract】 Objective To investigate the presence of HPV infection of oral mucosa proliferative lesions in children and determine the associations of HPV types with oral mucosa lesions in children. Methods Immunohistochemical method and in situ hybridization techniques were applied to detect human papillomavirus (HPV) infection in biopsies taken from clinical lesions in oral mucosa of 30 children. Results The most frequent lesions detected were SCP (66.7%), followed by CA and FEH. The HPV viral antigen was present in 73.3%(22/30)of the oral benign epithelial proliferative lesions in children. A high frequency HPV was found in CA (6/6) and SCP (15/20) by means of IHC. In the ISH positive case, high risk HPV 16/18 was observed in 77.3%(17/22). Conclusion This study demonstrates a high prevalence of HPV infection in children′s oral mucosa proliferative lesions, and high-risk HPV16/18 are predominant in children′s oral mucosa proliferative lesions.更多还原