【摘要】 目的　探讨结核性胸膜炎患者单个核细胞DNA氧化损伤和脂质过氧化的程度。方法采用单细胞凝胶电泳的方法检测 2 8例结核性胸膜炎患者 (试验组 )胸腔积液和外周血单个核细胞DNA损伤及 2 5名健康人 (对照组 )外周血单个核细胞DNA损伤 (以彗星率表示 ) ;采用菲罗啉比色法检测患者胸腔积液和血浆总抗氧化能力及健康人血浆总抗氧化能力 ;硫代巴比妥酸比色法检测患者及健康人血浆丙二醛 (MDA)含量。组间差异显著性比较采用t检验。结果　试验组胸腔积液单个核细胞彗星率为 (4 1 3± 14 5 ) % ,高于其外周血单个核细胞的 (2 1 2± 4 2 ) % (P <0 0 1) ;胸腔积液总抗氧化能力为 (5 172± 1195 )U/L ,显著低于其血浆的 (86 5 6± 15 92 )U/L(P <0 0 1)。胸腔积液单个核细胞彗星率与总抗氧化能力呈负相关 (r=- 0 4 2 5 ,P <0 0 5 )。试验组外周血单个核细胞彗星率及血浆MDA含量为 (2 1 2± 4 2 ) %和 (8 2 5± 1 37) μmol/L ,分别高于对照组的 (8 9± 3 7) %和(4 4 6± 0 93) μmol/L(P均 <0 0 1) ;试验组血浆总抗氧化能力为 (86 5 6± 15 92 )U/L ,显著低于对照组的 (10 6 10± 1399)U/L (P <0 0 1)。试验组血浆总抗氧化能力分别与外周血单个核细胞彗星率、血浆MDA含量呈负相关 (r分别为 - 0 4 38、-更多还原

【Abstract】 Objective To explore the levels of mononuclear cell oxidative DNA damage and lipid peroxidation in patients with tuberculous pleurisy. Methods The mononuclear cell DNA damages in pleural effusion and peripheral blood of 28 patients with tuberculous pleurisy and in peripheral blood of 25 healthy persons were detected by single cell gel electrophoresis(comet%). The levels of total antioxidative capacity (TAC) in supernatant of pleural effusion and blood plasma of 28 patients and in blood plasma of 25 healthy persons were measured by o-phenanthroline colorimetric analysis. The contents of malondialdehyde (MDA) of blood plasma from 28 patients and from 25 healthy persons were measured by thiobarbituric acid colorimetric analysis. The differences between groups were analyzed by t test. Results The comet percentage of mononuclear cells in pleural effusion from patients with tuberculous pleurisy was (41.3±14.5)%, which was significantly higher than that in the peripheral blood (21.2±4.2)% ( P<0.01); The level of TAC in supernatant of pleural effusion was (5 172±1 195) U/L, which was lower than that in the blood plasma (8 656± 1 592) U/L ( P<0.01). There was a negative correlation between the comet percentage of mononuclear cells and the level of TAC ( r=-0.425, P<0.05 ) in pleural effusion. The comet percentage of mononuclear cells in peripheral blood and the content of MDA in blood plasma from patients were (21.2±4.2)% and (8.25±1.37) μmol/L, which were significantly higher than those from the controls (8.9±3.7)% and (4.46±0.93) μmol/L respectively ( P<0.01); The level of TAC in blood plasma was (8 656± 1 592) U/L, which was lower than that from the controls (10 610± 1 399) U/L ( P<0.01). There were negative correlations between the level of TAC in blood plasma and thecomet percentage of mononuclear cells in peripheral blood or the content of MDA in blood plasma ( r=-0.438, -0.413, P<0.05), and there was a positive correlation between the comet percentage of mononuclear cells in peripheral blood and the content of MDA in blood plasma ( r=0.899, P<0.01). Conclusion There is oxidation/antioxidation imbalance in patients with tuberculous pleurisy, namely oxidative stress, with inadequate total antioxidative capacity, particularly in the diseased pleural cavity.更多还原