【摘要】 目的　探讨巨噬细胞在感染结核分支杆菌后氮氧化物 (NO)的产生和细胞因子表达的差异 ,了解结核感染的免疫应答过程 ,探讨死菌苗作为新的结核候选疫苗的可行性。方法　用ELISA和RT PCR方法比较研究巨噬细胞在感染结核分支杆菌后NO的产生和细胞因子表达的差异。结果活的结核分支杆菌H3 7Rv 能显著诱导巨噬细胞产生NO、IL 1、IL 12、IL 18、TNF α以及iNOS的表达。细菌数对NO的产生和细胞因子的表达也有很大的影响。结论　活的结核分支杆菌H3 7Rv 能显著诱导巨噬细胞产生NO和细胞因子 ,而产生有利于宿主的免疫应答。同样量的死结核分支杆菌H3 7Rv 不能显著诱导巨噬细胞产生NO和细胞因子 ,不宜作为新的结核候选疫苗。更多还原

【Abstract】 Objective To study nitric oxide (NO) production and cytokine expression by macrophages infected by M. tuberculosis H_ 37 R_v, and to compare the difference between dead and live M. tuberculosis in the induction of immune responses, and thus to show if dead bacteria could be a possible candidate for new vaccines. Methods Reverse transcription-polymerase chain reaction (RT-PCR) and ELISA were used to measure the production of NO and cytokines in macrophages infected by H_ 37 R_v. Results Macrophages infected by viable M. tuberculosis produced more NO, IL-1, IL-12, IL-18, TNF-α and inducible nitric oxide synthases (iNOS), as compared with macrophages infected by dead bacteria. The number of bacteria was also an important factor determining the production of NO and cytokines. Conclusions Viable M. tuberculosis H_ 37 R_v can induce the activation of macrophages and the production of more NO and cytokines which play important roles in the host immune response. Heat-killed M. tuberculosis H_ 37 R_v failed to induce activation of macrophages and the production of NO and cytokines, which makes it unlikely to be a candidate for vaccine development.更多还原