【摘要】 目的　萎缩性胃炎是癌前疾病之一 ,常为非萎缩性胃炎演变的后果。应用生物芯片技术观察萎缩性胃炎和非萎缩性胃炎胃黏膜组织基因表达谱的差异 ,探讨萎缩性胃炎发生的分子生物学机制。方法　连续登记在门诊接受胃镜检查的萎缩性胃炎和非萎缩性胃炎患者 2 2 7例 ,男 14 3例 ,女 84例 ,平均年龄 4 8.6岁 (16～ 72岁 )。胃镜检查时获取胃体和胃窦黏膜活检标本进行病理学检查 ,其余活检黏膜组织液氮冻存。根据病理学检查结果将冻存的组织标本分成萎缩性胃炎组和非萎缩性胃炎组 ,分别抽提两组组织的总RNA ,逆转录制备荧光cDNA探针 ,用含有 84 6 4条人类体细胞基因模板的表达谱cDNA芯片进行杂交实验。结果　与非萎缩性胃炎相比 ,萎缩性胃炎的活检胃黏膜组织中 16 5项基因的表达水平上调 2倍以上 ,4 6 0项的表达水平下调 5 0 %以上。表达下调者占 73.6 %。结论　基因表达谱生物芯片技术可以获得萎缩性胃炎胃黏膜组织基因在表达水平上改变情况的较全面的信息 ;萎缩性胃炎涉及多基因在表达水平上的改变。更多还原

【Abstract】 Objective Atrophic gastritis, as a pre-cancerous condition of gastric cancer, is developed from non-atrophic gastritis.The aims of this study was to compare the gene expression profiles between atrophic gastritis and non-atrophic gastritis using cDNA microarray and to explore the molecular mechanisms in the development of atrophic gastritis. Methods Endoscopy and biopsy were performed consecutively in 227 patients (143 male, 84 female, age range between 16-72 years, with average age 48.6 years). Diagnosis of non-atrophic gastritis and atrophic gastritis was made according to histological examination, and 120 patients were in non-atrophic group and 107 in atrophic group. The total RNA was extracted from the biopsy specimens of two groups with Trizol reagent. cDNA microarray consisting 8 464 human genes (HGEC-80s kit) was used, and labeled cDNA with fluorescence probes were hybridized to microarray, and then the comparison of gene expression profiles was made between atrophic and non-atrophic gastritis. Results It was identified that in gene expression profiles of atrophic gastritis there were 165 genes showing a greater than 2-fold increase and 460 genes showing a greater than 50% decrease as compared to non-atrophic gastritis. RT-PCR analysis revealed similar results as in DNA microarray. Conclusions There is different expression in many genes between the gastric mucosal cells of non-atrophic and atrophic gastritis mucosa. Many genes expression profiles are involved in atrophic gastritis that would be of help in further understanding the development of atrophic gastritis.更多还原