【摘要】 目的 观察乙型肝炎表面抗原（HBsAg）与粒细胞巨噬细胞集落刺激因子（GM CSF）融合表达质粒诱导特异性免疫应答的效果。方法 以HBsAg与GM-CSF融合表达质粒DNA免疫BALB/C小鼠,用酶链免疫吸附方法检测质粒诱导BALB/C小鼠产生抗HBs及脾细胞经HBsAg体外刺激后分泌白细胞介素（IL）-2、4及γ干扰素（IFNγ）的水平;并用²¹Cr释放法检测HHsAg特异性细胞毒性T淋巴细胞（CTL）反应。结果 各组小鼠加强免疫后血清抗体水平（P/N值）A组（PcDNA3.1-S）16.1+20.3,B组（PcDNA3.1 GM-CSF-S）28.3±19.7,F组（重组酵母乙型肝炎疫苗）29.5±21.5,C组（PcDNA3.1S-GM-CSF）0.3±0.0,D组（PcDNA3.1—S+PcDNA3.1-GM-CSF）4.5+5.9,E组（PcDNA3.1—GM-CSF）0.9±1.2,G组（PcDNA3.1）及H组（PBS）为阴性（F-4.176,P<0.01）;IL-2分泌水平A组（26.6±1.9）、B组（56.0±2.2）、C组（4.3±1.2）、D组（33.5±1.7）、F组（3.5+1.4）、F组（7.5±2.0）、G组（1.5±0.7）,H组未检测到IL-2分泌（F=31.188,P<0.01）;IFN-γ分泌水平A组（64.0±7.5）、B组（139.0±17.0）、C组（11.0±5.0）、D组（53.0±9.5）、E组（9.0±3.0）、F组（13.0±2.0）、G组(8.0±2.0,P（0.05）,H组未检测到IFN—γ泌（F=31.796,P<0.01）;HBsAg特异性CTL活性,效靶比为30:1及10:1时各组音有差异有显著性(F值分别为26.891、12更多还原

【Abstract】 Objective To investigate the improvement of specific immune responses induced by plasmid coexpressing hepatitis B surface antigen (HBsAg) and granulocyte-macrophage colony stimulating factor (GM-CSF). Methods All Balb/c (H-2d) mice were immunized with pGM-CSF/S, pS/GM-CSF, pS or control plasmids. 4 weeks later, anti-HBs titer and the levels of IL-2, IL-4 and IFN- y in the supernatant of splenocytes were detected using enzyme-linked immunosorbent assay (ELJSA), and HBsAg-specific cytotoxic T lymphocytes (CTL) activity was measured with a 51Cr release assay, using P815/S transfectants as target cells. Results The anti-HBs antibody titers in the serum, the levels of IL-2 and IFN- y , and the CTL aclivity in pcDNA3.1-GM-CSF-S immuned mice were higher than those in PcDNA3.1-S immunized mice (F = 4.176, P <0.01; F= 31.188, P < 0.01; F = 31.796, P < 0.01; F < 26.891, P < 0.01). Conclusion It will improve the specific immune responses induced by HBsAg DNA vaccine after it is binded to the gene of GM-CSF.更多还原