【摘要】 目的 通过前S2合成肽对肝细胞癌（HCC）组织中浸润T淋巴细胞亚群表达状况的分析,探讨前S2合成肽是否能改变乙型肝炎病毒（HBV）导致的HCC组织中浸润T淋巴细胞亚群表达的百分数和CD₄⁺/CD₈⁺比值,达到特异性治疗HCC。方法 用Merrifield固相化学合成法合成HBV中前S2抗原性最强的P_120～146多肽段。测序后,用0.01mol/L磷酸盐缓冲液溶解除菌后作为抗原。选择12例术后病理诊断为HCC,术前血清经酶联免疫吸附法测得HBsAg、HBeAg、抗—HBC阳性和HBV DNA阳性,且HBsAg在HCC组织中表达为阳性病例作为研究对象。在96孔培养板上,每孔加入经密梯度离心获得的单个核细胞悬液100μl（1×10⁶细胞）,设每孔含量为1μg、5μg、10μg、白细胞介素-2 500U和阴性对照共5组,每组均设3复孔,作用7d后制片,采用APAAP法观察CD₃⁺、CD₄⁺、CD₈⁺的百分数和CD₄⁺/CD₈⁺的比值变化。结果 发现在含有前S2合成肽5μg/孔组中,CD₄⁺表达明显增加（34.1±3.2）,与自身对照（29.3±3.5）相比差异有显著性（t=3.508,P<0.01）,CD₄⁺/CD₈⁺比值（1.19±0.43）与自身对照（0.81±0.41）相比差异有显著性（t=2.235,P<0.05）。在白细胞介素-2组中,CD₃⁺表达有增加,与自身对照相比有差异,其它各组T淋巴细胞亚群几乎处于不变化的状态。结论 在适当的剂量?更多还原

【Abstract】 Objective To explore whether PreS2 can change the percentage of T lymphocyte subgroups and the ration of CD4+/CD8+ in hepatocellular carcinoma (HCC) caused by HBV. Methods The P120-146 region composed by the way of Merrifield, which was the most intensive antigen in PreS2 peptides, served as the antigen after dissolved in 0. 01 mol/L PBS. 12 patients were chosed as the subjects, who were pathologically diagnosed as HCC after operation, were HBsAg-, HBeAg-, anti-HBc, and HBV DNA positive in serum, and expressed HBsAg in HCC tissue. The monocytes were isolated and cultured in 96 microplate with 1 x 106 cells in every well, then the PreS2 synthetic peptides was added in at the doses of 1μg, 5μg, and 10μg, also IL-2 with 500 U was added in. Seven days later, the percentage of CD3+, CD4+, CD8+, and the ratio of CD4+/CD8+ were detected. Results It was found that the percentage of CD4+ increased significantly (t = 3.508, P < 0.01), and the ratio of CD4+/CD8+ decreasedly obviously (t = 2.235, P < 0.05) in the 5μg PreS2 synthetic peptides group, compared with those in the control group. The percentage of CD3+ rised markedly in the IL-2 group, compared with that in the control group. Conclusion With proper doses, PreS2 is capable of changing the expression of T lymphocyte subgroups in HCC tissue, increasing the percentage of CD4+ obviously and changing the motionless state of CD8+, to make the carcinoma cell killed through the action of CD4+ and CD8+.更多还原