【摘要】 目的　探讨γ射线对大鼠血管平滑肌细胞 (VSMCs)抑制作用的机理。方法　对大鼠VSMCs进行原代培养 ,经3H TdR掺入法观察γ射线对VSMCs增殖情况的影响。应用流式细胞仪检测γ射线对VSMCs细胞周期的影响。采用WesternBlot技术检测γ射线对VSMCsp53、细胞周期素D(cy clinD)和增殖细胞核抗原 (PCNA)表达的影响。结果　γ射线对VSMCs增殖的抑制作用呈剂量依赖性。γ射线照射可诱导VSMCs出现G1 期阻滞 ,在照射后一定时间内会导致p53表达增强 ,cyclinD和PCNA表达减弱。结论　γ射线可抑制VSMCs增殖 ,其机制可能主要是γ射线诱导VSMCs出现G1 期阻滞 ,抑制VSMCs进行有丝分裂。在这个过程中p53、cyclinD和PCNA等分子起到一定作用更多还原

【Abstract】 Objective To investigate the mechanism of the inhibitory effect of γ-ray irradiation on rat vascular smooth muscle cells(VSMC). Methods The ef fect of γ-ray irradiation on proliferation of VSMC was observed by 3?H-TdR incor poration. After γ-ray irradiation, the VSMC cell cycle change was detected b y flo w cytometry. The expression of p53, cyclin D and PCNA was investigated by Wester n Blot. Results The inhibitory effect of γ-ray irradiation on VSMC proliferati on was dose-dependent. After γ-ray irradiation, VSMC was arrested in G 1 st age, w ith the expression of p53 increased but the expression of cyclin D and PCNA decr eased. Conclusions γ-ray irradiation can inhibit the proliferation of VSMC. T he main mechanism is probably due to the induction of cell cycle arrest and inhi bition of the VSMC mitosis ,during which process, p53,cyclin D and PCNA all pla y an important role .更多还原