【摘要】 目的　探讨先兆子痫患者血清中激活素A和卵泡休止素水平及其mRNA在胎盘组织中的表达 ,及其与先兆子痫发病的关系。方法　 2 0例足月妊娠先兆子痫孕妇作为先兆子痫组 ,2 0例足月妊娠血压正常孕妇作为对照组。应用酶联免疫吸附试验 (ELISA)检测两组孕妇血清中激活素A和卵泡休止素水平。应用半定量逆转录 聚合酶链反应 (RT PCR)技术检测两组孕妇分娩后胎盘组织中激活素AmRNA和卵泡休止素mRNA的相对表达强度。将两组孕妇的胎盘组织激活素AmRNA的表达强度与血清激活素A水平进行直线相关分析。结果　 (1)先兆子痫组孕妇血清中激活素A水平为 (33 7± 6 6 ) μg/L ,明显高于对照组的 (9 9± 2 1) μg/L(P <0 0 1)。先兆子痫组孕妇血清中卵泡休止素水平为 (5 1± 0 6 ) μg/L ,与对照组的 (4 7± 0 3) μg/L比较 ,差异无显著性 (P >0 0 5 )。 (2 )先兆子痫组胎盘组织中激活素AmRNA为 1 11± 0 2 1,明显高于对照组的 0 6 1± 0 17(P <0 0 1)。先兆子痫组胎盘组织中卵泡休止素mRNA为 0 5 7± 0 31,与对照组的 0 5 4± 0 2 7比较 ,差异无显著性 (P >0 0 5 )。(3)在先兆子痫组和对照组孕妇中 ,血清中激活素A水平与胎盘组织激活素AmRNA相对表达强度呈正相关 [相关系数 (r) =0 89,P <0 0 1]。结论更多还原

【Abstract】 Objective The purpose of this study was to determine the relationship of maternal serum activin A,follistatin and their mRNA expression in placenta with preeclampsia at term. Methods Twenty women with preeclampsia were enrolled in this study.Twenty healthy pregnancies matched for gestational ages were recruited as control group.Maternal serum activin A and follistatin in two groups were compared by using enzyme linked immunosorbent assay (ELISA).Placental tissues were obtained from preeclampsia and healthy control groups for analysis of activin A mRNA and follistatin mRNA expression by using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).Linear correlation analysis was used to explore the relationship between placental avtivin A mRNA and maternal serum activin A concentrations. Results (1) Maternal serum concentrations of activin A are significantly increased in preeclampsia group (33.7±6.6)μg/L compared with control group (9.9±2.1)μg/L ( P < 0.01). Maternal serum follistatin levels were not different between preeclampsia group (5.1±0.6)μg/L and control group (4.7±0.3)μg/L( P >0.05).(2) The placentas of preeclampsia group expressed activin A mRNA levels at significantly higher levels 1.11±0.21 than that of control group 0.61±0.17 ( P <0.01).The expression of follistatin mRNA were not different between preeclampsia group 0.57±0.31 and control group 0.54±0.27( P >0.05).(3) In preeclampsia and control groups,maternal serum activin A concentrations had a significant positive correlation with activin A mRNA expression in placentas ( r =0.89, P <0.01). Conclusion These results suggest that activin A is increased in women with preeclampsia,the elevated levels of activin A due to increased placental production.更多还原