【摘要】 目的　探讨妊娠期妇女血清和胎盘组织中卵泡休止素 (FS)水平变化及意义。方法　采用FS特异双抗体夹心酶联免疫吸附试验 ,检测早期妊娠、足月妊娠妇女和生理月经期妇女的血清FS水平 ;采用逆转录聚合酶链反应 (RT PCR)法、半定量检测FSmRNA在足月妊娠妇女胎盘及卵巢组织中的表达。结果　 (1)足月妊娠妇女血清FS水平为 (180± 2 6 ) μg/L ,明显高于早期妊娠妇女的(4.4± 2 .1) μg/L ,两者比较 ,差异有极显著性 (P <0 .0 0 1) ;也明显高于生理月经期妇女的 (1.5± 2 2 ) μg/L ,两者比较 ,差异有极显著性 (P <0 .0 0 1)。 (2 )足月妊娠妇女胎盘组织FSmRNA呈强阳性表达 ,生理月经期妇女的卵巢组织FSmRNA呈阳性表达 ,足月妊娠妇女的卵巢组织FSmRNA呈弱阳性表达。 (3)RT PCR检测显示 ,足月妊娠妇女的胎盘组织FSmRNA的表达水平 (0 .8± 0 .4 )高于其卵巢组织 (0 .4± 0 .3) ,两者比较 ,差异也有显著性 (P <0 .0 5 ) ;但低于生理月经期妇女的卵巢组织 (0 .93± 0 .13) ,两者比较 ,差异有显著性 (P <0 .0 5 )。结论　 (1)妇女生理月经期卵巢组织可分泌FS ,并有少量的FSmRNA表达。但在妊娠期卵巢分泌FS的功能受到抑制。 (2 )足月妊娠妇女的血清FS主要来源于胎儿 胎盘单位。FS在妊娠过程中对胚胎发育及胎盘功能?更多还原

【Abstract】 Objective To investigate the possible origin and role of follistatin (FS) in pregnancy Methods The maternal serum FS levels in menstrual cycle, first trimester (8～12 weeks) and term (38～41 weeks) were determined by a specific two site enzyme linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction (RT PCR) method was utilized to semi quantitatively determine FS mRNA levels in the ovaries and placentas Results Maternal serum FS level at term was significantly higher than that in the first trimester of pregnancy and that in normal women during the menstrual cycle(180±26)μg/L ,(4.4±2.1)μg/L, (1.5±2.2)μg/L , (respectively P <0.001). Both placentas and ovaries expressed FS mRNA. Semi quantitative RT PCR showed that the expression levels of FS mRNA in placenta were higher than that in ovary of same period, but lower than that in ovary of menstrual phase (0 8±0.4,0.4±0.3,0.93±0.13, respectively P <0.05) Conclusion Fetal placental unit may be the major source of maternal serum FS. The relatively higher levels of FS in the placentas at term may reflect important roles in modulating the development of embryo and the function of placenta.更多还原