【摘要】 目的　观察幼年哮喘大鼠嗜酸细胞 (EOS)凋亡及凋亡基因fas和bcl 2的mRNA表达及牛膝多糖对其的作用。方法　清洁级雄性SD大鼠随机分为 5组 :正常对照组 (C) ,哮喘组 (A) ,牛膝多糖治疗组 (T1、T2、T3 )。采用卵清白蛋白复制幼年大鼠哮喘模型。用寡核苷酸探针原位杂交法和TUNEL法检测各组大鼠fas和bcl 2mRNA在气道壁EOS上的表达和气道壁EOS的凋亡率。结果 ( 1)C组大鼠肺组织EOS上fasmRNA的表达率为 ( 73 2± 11 9) % ,A组为 ( 4 3 4± 10 0 ) % (P <0 0 1) ;在T1、T2、T3组分别为 ( 59 0± 8 1) %、( 57 5± 9 6) %、( 76 2± 2 7) % ,与A组相比差异均有显著性 (P <0 0 5,0 0 1)。C组大鼠EOSbcl 2mRNA的表达率为 ( 4 7 9± 8 7) % ,在A组为 ( 67 4± 7 3 ) % (P <0 0 1) ;T1组的bcl 2mRNA表达率为 ( 57 7± 12 7) %、T3组为 ( 57 3± 6 8) % ,与A组相比差异均有显著性 (P均 <0 0 5) ,T2组与A组相比差异无显著性。 ( 2 )A组EOS凋亡率为 ( 5 3± 2 2 ) % ,而C组为( 15 9± 2 4) % (P <0 0 1)。T1、T3组EOS凋亡率分别为 ( 8 7± 2 9) %、( 9 8± 2 2 ) % ,与A组相比差异均有显著性 (P均 <0 0 5)。结论　哮喘大鼠气道EOS表现为fas、bcl 2基因表达的明显改变及凋亡率明显下降。ABPS可调节EOS上更多还原

【Abstract】 Objective Asthma is a chronic respiratory tract disorder characterized by airway hyperreaction (AHR), persistent airway inflammation, high serum IgE, overproduction of IL 4, IL 5 and IL 13 by allergen specific Th2 cells The morbidity and mortality of asthma have continued to increase despite the use of currently available therapeutic agents The reputed effects of traditional Chinese medicines (TCMs) have led to increasing use of TCMs for treatment of asthma throughout the world The aims of this study were to investigate in asthma model of young rat the mRNA expressions of apoptotic gene fas and bcl 2, eosinophils (EOS) apoptosis in airway, and effects of achyranthes bidentata polysaccharides (ABPS), a group of polysaccharides extracted from TCM Achyranthes bidentata blume, on treatment of asthma Methods Fifty Sprague Dawley (SD) rats were divided into five groups,10 rats per group Asthma in rats was induced by intraperitioneal sensitization and challenge with nebulized ovalbumin (OVA) A pretreatment with ABPS [50 mg/(kg·d)] was done according to three different schedules: consecutively 3 days at sensitization (T1), at challenge (T2) or both of the two periods (T3) Sham treated rats (A) and naive rats (C) served as controls The animals were sacrificed 24 hours after the last challenge The mRNA expression of bcl 2 and fas in eosinophils presenting in airway and the apoptosis of eosinophils in airway were assessed by using in situ hybridization with oligonucleotide probe and TUNEL methods, respectively Results (1) Twenty four hours after the last antigen challenge, the mRNA expression of fas in eosinophils presenting in airway significantly decreased in group A[(43 4±10 0)%] compared with that in group C[(73 2±11 9)%] ( P <0 01) ABPS could increase the fas mRNA expression significantly in all the three groups [(59 0±8 1) %, (57 5±9 6) %, (76 2±2 7) %], compared with that in group A ( P <0 05, P <0 05, P <0 01, respectively) The expression of the bcl 2 mRNA in group C was (47 9±8 7)%, it was elevated to (67 4±7 3)% in group A ( P <0 01) The expression of the bcl 2 mRNA in ABPS treated T1 and T3 groups was significantly lowered [(57 7±12 7)%, (57 3±6 8)% , P <0 05], but not in T2 group [(72 4±6 7)%] (2) In group A ,the EOS presenting in the airway increased significantly, but there were few apoptotic EOS; the percentage of apoptotic eosinophil was distinctly lower in group A than that in group C [(5 3±2 2)% vs (15 9±2 4)%, P <0 01] Compared with that in group A, the eosinophil apoptosis ratio in those ABPS treated groups T1, T3 was evidently elevated [(8 7±2 9)%, (9 8±2 2)%, P <0 05, P <0 05], but ABPS treated at challenge (T2) could not change the eosinophil apoptosis ratio significantly ( P >0 05) Conclusion (1) In asthmatic rat, the expressions of the genes fas and bcl 2 mRNA in EOS were changed evidently and the ratio of EOS apoptotosis reduced greatly (2) ABPS could enhance the apoptosis of EOS by upregulating the expression of the genes fas and bcl 2 mRNA更多还原