【摘要】 目的　探讨Toll样受体 2 (Toll likereceptor 2 ,TLR 2 )及其Toll样受体 4(Toll likereceptor 4,TLR 4)mRNA在慢性鼻窦炎患者和健康成人鼻黏膜上皮细胞中的表达 ,分析TLR 2和TLR 4在鼻黏膜天然免疫中的作用。方法　应用核酸分子原位杂交技术检测 3 0例慢性鼻窦炎患者和 2 1例健康成人鼻黏膜上皮细胞中TLR 2和TLR 4mRNA的表达。结果　TLR 2和TLR 4mRNA在 3 0例慢性鼻窦炎上皮细胞中均有表达 ,且分别与对照组间差异有显著性意义 (t =8 60 5,P <0 0 0 0 5,t =9 0 50 ,P <0 0 0 0 5)。结论　鼻黏膜上皮细胞中可以产生TLR 2和TLR 4,表明鼻黏膜上皮不仅是单纯的物理屏障 ,更可以通过Toll样受体对病原微生物进行识别 ,以直接启动有效的抗感染机制。更多还原

【Abstract】 Objective To understand the expression of Toll-like receptor-2(TLR-2) and Toll-like receptor-4(TLR-4) in epithelial cell of nasal mucosa, and to investigate the role of Toll-like receptors in the innate immunity of nasal mucosa Methods Specimens from 30 patients with chronic sinusitis and 21 healthy adults were examined by in situ hybridization for TLR-2 and TLR-4 mRNA Results All 30 samples of chronic sinusitis showed a stranger expression of TLR-2 and TLR-4 than in controls (t=8 605, P<0 000 5, t=9 050,P<0 000 5) Conclusion It is suggested that epithelia in nasal mucosa were not just a physical barrier, which could represent a novel defense mechanism of the host during bacterial infections through Toll-like receptors更多还原