【摘要】 目的　观察急性脑损伤后神经元凋亡现象。　方法　以大鼠急性弥漫性脑创伤模型为研究对象 ,采用光镜、电镜观察方法对模型的损伤情况进行组织、细胞形态观察 ;以DNA -梯形凝胶电泳、凋亡细胞原位末端标记法 (TUNEL)对急性脑损伤后鼠脑皮层、海马区神经元DNA损伤情况进行观察。　结果　大鼠致伤后 ,光镜下观察到神经元出现皱缩变性 ;电镜下观察发现 ,伤后2h可观察到神经元类凋亡 ,2 4h最为严重 ,持续到 7d ;DNA -梯形凝胶电泳显示伤后 2 4h海马及皮层区出现DNA梯状电泳 ;神经元TUNEL染色伤后 2h即可见 ,2 4h最为明显 ,7d时仍高于正常。结论　大鼠急性脑创伤后脑组织中存在神经元类凋亡现象 ,并在创伤后较长时间内持续存在更多还原

【Abstract】 Objective To observe neuronal apoptosis after acute brain trauma (ABT). Diffused brain trauma model was used to histologically and morphologically observe the neuronal damage by microscopy and electromicroscopy. The neuronal DNA injury in the brain cortex and hippocampus was detected by DNA Ladder gel electrophoresis and TUNEL stain. Results After impact, the shrunken and contracted degenerations were found in neurons under microscopy. Under the electromicroscopy, para apoptosis could be seen at the 2nd hour after impact, became the most distinct at the 24th hour and lasted 7 days. There appeared DNA ladder electrophoresis in the hippocampus and the cortex at the 24th hour after impact through DNA ladder gel electrophoresis. Positive TUNEL stain could be easily seen at the 2nd hour after impact, reached most distinct at the 24th hour and was still higher than normal at the 7th day. Conclusions Neuronal para apoptosis exists in the brain tissues after ABI and can last a long time.更多还原