【摘要】 目的　对福氏 2a志贺菌肠毒素ShET1和肠毒素ShET2进行基因分型 ,提高福氏 2a志贺菌同源克隆鉴定系统的分析水平。方法　对 93株分离自不同地区、不同时间的福氏 2a志贺菌用PCR法检测志贺菌肠毒素ShET1/ShET2基因并进行基因分型。结果　 93株福氏 2a志贺菌按志贺菌肠毒素ShET1/ShET2基因分为 4种基因型 ,即 12株ShET1(- ) /ShET2 (+) ,14株ShET1(+) /ShET2 (- ) ,5 9株ShET1(+) /ShET2 (+) ,8株ShET1(- ) /ShET2 (- )。结论　福氏 2a志贺菌肠毒素ShET1/ShET2基因PCR检测可用于福氏 2a志贺菌的初步筛检。更多还原

【Abstract】 Objective To identify homogenous clones of S.flexneri 2a, using gene distribution polymorphism of Shigella enterotoxinl and Shigella enterotoxin2 in a shigellosis outbreak by S.flexneri 2a. Methods 93 strains of S.flexneri 2a isolated from different areas, at different time were detected by Shigella enterotoxin1 and Shigella enterotoxin2 gene polymerase chain reaction to identify genetic type of S.flexneri 2a. Results The 93 strains of S.flexneri 2a were divided into 4 clones: ShET1(-)/ShET2(+)for 12 strains,ShET1(+)/ShET2(-)for 14 strains,ShET1(+)/ShET2(-)for 59 strains, ShET1(-)/ShET2(-)for 8 strains. Conclusions It is believed that Shigella enterotoxin1 and Shigella enterotoxin2 gene PCR analysis is a simple method for the primary screening of S.flexneri 2a.更多还原