【摘要】 目的　用基因微矩阵技术研究胸腺肽α1作用于人外周血淋巴细胞后 ,细胞内与抗感染相关的信号转导分子的基因表达谱变化。方法　取正常外周血 6 0 0ml分离淋巴细胞 ,实验组加Tα1(0 .1μg/ml)刺激 ,对照组不加Tα1。培养 4 8h后 ,收获细胞、抽提细胞总RNA并纯化mRNA。逆转录反应将对照组和实验组的cDNA分别标记上荧光分子Cy3和Cy5 ,与信号转导表达谱基因微矩阵杂交后激光扫描、图像分析。结果　筛选出 6 1种Cy5 /Cy3比值大于 2或小于 0 .5的差异表达基因 ,其中与感染相关的有CAML ,PI3 K ,TSP2 ,HM 74 ,PIAS ,PTP ,PSTP等 7种基因表达上调 ,MEK2 ,MAP3K ,Lok等 3种基因表达发生下调。结论　Tα1的直接抗感染作用与这些信号转导分子的基因表达变化有关。更多还原

【Abstract】 Objective To study the effect of thymosin alpha 1 on expression profiles of signal transductors involving anti-infection in human peripheral blood lymphocytes. Methods Human peripheral blood lymphocytes isolated from three health donors were cultured with PHA（1 μg/ml） for 48 hours and then divided into experimental group and control group. The experimental group was stimulated by Tα₁ （0.1 μg/ml） additionally. Thereafter, the total RNA were extracted by TRIzol TM and further purified for mRNA. The fluorescent cDNA probes were prepared by labeling experimental group mRNA and control mRNA with Cy5-dUTP and Cy3-dUTP respectively through reverse transcription. The microarray was then hybridized against the cDNA probe mixture and the fluorescent signals were laser scanned and analyzed. Results According screening standard, there were 61 genes differentially expressed after stimulation with Tα₁ with the Cy5/Cy3 ratio >2 or <0.5. Among those genes with functions related to anti-infection, CAML,PI3-K,TSP2,HM74,PIAS,PTP,PSTP genes were up-regulated, MEK2,MAP3K,Lok genes were down-regulated. Conclusions The anti-infection function of Tα₁ were related to these up-regulated and down-regulated genes.更多还原