【摘要】 目的　了解乙型肝炎患者血清中乙型肝炎病毒 (HBV)DNAX区核苷酸序列的变异情况。方法　采用聚合酶链反应 (PCR)扩增 2 4例乙型肝炎患者血清HBVDNAX区产物 ,并直接测序进行分析。结果　 2 4例患者血清中HBVDNAX区都有程度不等的点突变 (2～ 15 ) ,11例同时具有nt176 2 (A→T)、nt176 4(G→A)发生变异 ,8例患者同时在nt16 36～nt1741几处位点发生变异。结论HBVDNAX区核苷酸位于nt176 2、nt176 4双位点变异与HBeAg阴性表型有关。更多还原

【Abstract】 Objective To investigate the mutations of HBV X region nucleotide sequence in the patients with chronic hepatitis B. Methods X region fragments of HBV DNA from 24 patients were amplified by polymerase chain reaction and sequenced. Results There were 2 15 point mutations in X region from the 24 hepatitis patients studied. The A to T mutation at nt1762 and G to A mutation at nt 1764 were found in 11 cases. The combined mutation at nt1636-1741 were found in 8 cases.Conclusions The results indicated the combined mutations of nucleotide(nt) 1762 and 1764 was associated with HBeAg(-) in the patients with chronic hepatitis.更多还原