【摘要】 目的 :为了探讨几种理化因子对贯叶金丝桃愈伤组织中金丝桃素含量的影响。方法 :采用高效液相色谱法和植物组织培养的方法。结果与结论 :当硝态类氮和铵态类氮的比例 3∶1时贯叶金丝桃愈伤组织的生长量提高至 1.6倍 ,金丝桃素的合成量也略有提高 ;加入甘露 (0 .1～ 0 .2mol·L-1时 )金丝桃素的合成量有很大的提高 ;加入PVPP ,PVP等防褐化的物质能促进贯叶金丝桃愈伤组织的生长。更多还原

【Abstract】 Objective:To study the effect of several factors on the quantity of hypericin in H. perforatum callus. Method: High efficiency liquid phase chromatography and plant tissue culture were applied. Result and Conclusion:When the ratio of nitro-nitrogen to amina-nitrogen is 3∶1, the callus biomass is 1.6 -fold and the synthetic mass of hypericin rises. 0.1～0.20 mg·L -1 mannose improvesthe content of total hypericin. The addition of PVP or PVPP can promote improvement of the growth and biosynthesis of callus.更多还原