【摘要】 目的 :观察中药益心酮片调控细胞膜钙通道的特性 ,从细胞和亚细胞水平探讨其改善心肌缺血再灌注损伤的作用机理。方法 :采用大鼠主动脉平滑肌细胞模型 ,用放射性4 5Ca跨膜流动测定技术分别观察益心酮片对溢流钙通道 (LC)、受体调控钙通道 (ROC)和电压依赖钙通道 (PDC)Ca2 + 内流的影响。结果 :益心酮片对LCCa2 + 内流无明显影响 ,对ROC和PDCCa2 + 内流有明显抑制作用。结论 :益心酮可以抑制慢通道Ca2 + 内流 ,且抑制作用呈现剂量依赖关系更多还原

【Abstract】 Objective:To explore the protecting mechanism of Yixintong for heart ischemia reperfusion injury at cellular and subcellular levels, by observing the effects of Yixintong on three kinds of calcium channels. Method:The effects of Yixintong on Ca 2+ influx on leak calcium channel, receptor operationg calcium channel (ROC) and pulse dependent calcium channel (PDC) were observed respectively, by using rat aortic smooth muscle cell and radioactive 45 Ca technique. Result:Yixintong has no effects on leak calcium channel, but can inhibit the Ca 2+ influx in ROC and PDC significantly. Conclusion:Yixintong can inhibit the Ca 2+ influx in slow channel in a dose dependent manner.更多还原